getpdf  NLM PubMed Logo  https://doi.org/10.17113/ftb.57.02.19.5964      


Nitric Oxide Synthesis Inhibition and Anti-Inflammatory Effect of Polypeptide Isolated from Chicken Feather Meal in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages   


Aurachorn Inkanuwat1orcid tiny, Romteera Sukaboon2orcid tiny, Onrapak Reamtong3orcid tiny, Pravit Asawanonda4orcid tiny, Ake Pattaratanakun5orcid tiny, Tanatorn Saisavoey6orcid tiny, Papassara Sangtanoo6orcid tiny and Aphichart Karnchanatat6*orcid tiny  


1Technopreneurship and Innovation Management Program, Graduate School, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand 
2Program in Biotechnology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand 
3Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok 10400, Thailand 
4Department of Medicine, Faculty of Medicine, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand 
5Department of Marketing, Faculty of Commerce and Accountancy, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand 
6Research Unit in Bioconversion/Bioseparation for Value-Added Chemical Production, Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand   



Article history
Received: 2 August 2018  
Accepted: 26 March 2019 
cc  



Key words
nitric oxide, anti-inflammatory activity, chicken feather meal, protein hydrolysate, polypeptide, macrophage RAW 264.7  



Summary
Nitric oxide (NO) plays a key role in the pathogenesis of inflammation and has been implicated in endotoxin-induced tissue injury. Chicken feather meal is a rich source of amino acids that may serve as a peptide hydrolysate to inhibit NO activity. Anti-inflammatory hydrolysates of chicken feather meal were prepared and fractionated into five samples based on molecular mass. The smallest fraction (<0.65 kDa) exhibited the highest NO inhibitory activity without cytotoxicity towards macrophage RAW 264.7 cells. Further subfractions were sufficient to obtain amino acid sequences by Q-TOF LC-MS/MS ESI analysis. Of these, the SNPSVAGVR (885.97 Da) peptide and its corresponding pure synthetic peptide have inhibitory activity against NO production by RAW 264.7 cells (IC50=(55.2±0.2) mM) without cytotoxicity. Reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative real-time RT-PCR results revealed that the peptide of the obtained fraction reduced transcript expression levels of the pro-inflammatory cytokines iNOS, TNF-α, COX-2 and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells. These results suggest that the peptides derived from the chicken feather meal protein could potentially be used as a promising ingredient in functional foods or nutraceuticals against inflammatory diseases.    




*Corresponding author:  tel3  +6622188078  
                                           
fax2  +6622188069
                                          
  email3  Aphichart.K@chula.ac.th

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