Genetic and Molecular Diversity in Saccharomyces cerevisiae Natural Populations
D. Cavalieri1*, C. Barberio1, E. Casalone2, F. Pinzauti1, F. Sebastian1, R. Mortimer3, M. Poisinelli1
1Dipartimento di Biologia Animale e Genetica, Universita di Firenze, Via Romana 17, 50125 Firenze.
2Dipartimento di Scienze Biornedicho, UniversitAdi Chieti, via dei Vestini, 66100 Chieti,
3Department of Molecular and Cell Biology, University of California, Berkeley CA 94720, USA.
Received: September 9, 1997
Accepted: January 15, 1998
Saccharomyces cerevisiae, biodiversity, genetics, wine yeast, DNA fingerprint
Two Saccharomyces cerevisiae populations isolated from grapes collected in two geographically distinct Italian regions, Tuscany and Sicily, were partially characterized by a multimelhodological approach including genetic and molecular techniques. A total of 166 isolates, 61 from Sicily and 105 from Tuscany were characterized by random amplification of polymorphic DNA analysis. Preliminary residts, obtained on 36 strains that were not differentiated with the above mentioned technique, showed that genetic analysis combined with restriction fragment length polymorphism using the DC4 probe are the most powerful combination of methods. Using this approach we were able to show that 34 of the 36 strains zvere unique and the remaining two fell into one class. This demonstrates a high level of biodiversity in these populations.
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