getpdf

Basic Principles of the Use of Immunoaffinity Chromatography for Environmental Analysis

Berta Ballesteros, Maria-Pilar Marco


Department of Biological Organic Chemistry. C.I.D.-C.S.I.C., Jordi Girona 18-26, 08034-Barcelona, Spain 


Article history:

Received July 15, 1997
Accepted April 7, 1998

Key words:

pollutant, antigen/antibody interaction, intmlinosorbent, enviromental analysis

Summary:

Usual pollutant extraction procedures applied to environmental samples are not selective. Therefore, the analysis of trace-level of contaminants or their degradation products in complex matrices is often difficult because of the different polarities and low concentrations of the analytes. Additionally, very polar analytes are often not quantitatively retained by the most common solid phase extraction procedures. Immunoaffinity chromatography (IAC) is a separation method based on the specific and reversible interactions between the antigen and the antibody. Because the retention is directed by a different physico-chemical principle, IAC offers an interesting alternative to implement the existing extraction procedures. In this paper we will present the basic principles of the IAC separations. Based on the available literature, the most important criteria for immunosorbent preparation and the operational features of this kind of solid phase extraction method will be discussed. Slate of the art of the application of IAC for environmental analysis will be presented.  



*Corresponding author:          mpmqob@cid.csic.es
                                              34 3 4006100
                                              34 3 204 59 04

Search FTB


Follow us


 facebook 1 twitter bird_icon LI In Bug

 

QR Code


qrcode

We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information