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Glucose and Maltose Metabolism in MIG1-disrupted and MAL-constitutive Strains of Saccharomyces cerevisiae

Christopher J. L. Klein1, Lisbeth Olsson1*, Birgitte Ronnow2, Jørn Dalgaard Mikkelsen2, Jens Nielsen1


1
Center for Process Biotechnology, Department of Biotechnology, Building 223,
Technical University of Denmark, DK-2800 Lyngby, Denmark

2
Danisco Biotechnology, PO Box 17, Langebrogade 1, DK-1001 Copenhagen K, Denmark

Article history:

Received September 10, 1997

Accepted October 21, 1997

Key words:

Saccharomyces cerevisiae, baker's yeast, glucose repression, catabolite inactivation, A/I1G1, MAL, genetic engineering

Summary:

The alleviation of glucose control of maltose metabolism brought about by MIG1 disruption was compared to that by MAL overexpression in a haploid Saccharomyces cerevisiae strain. The sugar consumption profiles during cultivation of the wild type, single transformants and a double transformant in a mixed glucose-maltose medium revealed that the MAL-constitutive strains zvere more alleviated than the single MIGl-disrupted transformant. While all transformants exhibited higher maximum specific growth rates (0,24-0.25 h-1) in glucose-maltose mixtures than the wild type strain (0.20 h-1), the MAL-constitutive transformants grew even faster (0.27-0.30 h-1) in pure glucose medium than the wild type strain (0.24 h-1).


*Corresponding author:           lo@ibit.dtu.dk
                                               45 45 884 148

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