getpdf

Spot-Synthesis: Easy Multiple Preparation of Synthetic Peptides on Cellulose Membrane for the Analysis of Anti-Protein Antibodies 

Daniela Lucchesi, Paolo Rovero
*


CNR — Istituto di Mutagenesi e Differenziamento, Laboratorio di Sintesi Peptidica, Via Svezia 2A, 56124 Pisa, Italy


Article history:

Received July 9, 1997
Accepted September 30, 1997

Key words:

cellulose paper, epitope mapping, enzyme-linked irnmunosorbent assay (ELISA), multiple peptide synthesis, peptide libraries

Summary:

The SPOT synthesis is a novel technique for the simultaneous multiple synthesis of peptides covalenlly linked to cellulose paper, using conventional solid-phase synthesis strategy. The sets of paper-bound peptides thus obtained can be directly used in solid-phase assays, including ELISA. The technique is a powerful tool for the study of protein-interactions and has been widely applied to antibody characterization and epitope mapping studies. The SPOT technique provides a simple, economic and rapid access to large numbers of short peptide sequences or peptide mixtures which can be tested concomitantly with a single serum and repeatedly with many sera on the same membrane. Recent applications of SPOT synthesis to epitope mapping are reviewed in this report. 



*Corresponding author:    p.rovero@imd.pi.cnr.it

Search FTB


Follow us


 facebook 1 twitter bird_icon LI In Bug

 

QR Code


qrcode

We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information