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Screening for Extracellular Lipase Enzymes with Transesterification Capacity in Mucoromycotina Strains

Alexandra Kotogán, Brigitta Németh, Csaba Vágvölgyi, Tamás Papp and Miklós Takó*


Department of Microbiology, Faculty of Science and Informatics, University of Szeged,
Közép fasor 52, HU-6726 Szeged, Hungary

Article history:

Received June 3, 2013

Accepted January 30, 2014

Key words:

zygomycetes, tributyrin, wheat bran, p-nitrophenyl palmitate, transesterification

Summary:

In this study, 169 zygomycetes fungal strains including some cold-tolerant isolates
were screened for their extracellular lipolytic activity towards tributyrin. Nineteen of them were outstanding in their enzyme production as they developed the largest lipolytic halo around the colonies in plate tests. Mortierella alpina, M. echinosphaera, Mucor corticolus, Rhizomucor miehei, Rhizopus oryzae, Rh. stolonifer, Umbelopsis autotrophica, U. isabellina, U. ramanniana var. angulispora and U. versiformis were selected for further studies to characterise their lipolytic enzyme production in detail. In these assays, effect of Tween 80 and palm, soybean, sunflower, olive, extra virgin olive, wheat germ, corn germ, sesame seed, pumpkin seed and cottonseed oils on the enzyme activities was investigated, and wheat bran-based submerged and solid-state fermentations were also tested. Tween 80 and olive oil proved to be efficient inductors for lipolytic enzyme production, which was also enhanced when wheat bran was used as support. Addition of mineral salts and olive oil to the solid fermentation medium resulted in at least 1.5-fold increment in the enzyme activities of the crude extracts. Organic synthesis was also assayed by the selected lipases, in which enzymes from the fungi R. miehei, Rh. stolonifer and M. echinosphaera gave the best yields during transesterification reactions between p-nitrophenyl palmitate and ethanol.

 

 

*Corresponding author:        tako78@bio.u-szeged.hu
                                                          +36 62 544 516

                                            +36 62 544 823

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