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Prevention of β-Glucosidase Inhibition by High Molecular Mass Compounds During Enzymatic Wine Aroma Enhancement Using a Hollow Fiber Reactor

Alicia Graciela Cid*, Mirta Daz and Guillermo Ellenrieder


National Research Institute for Chemical Industry (National University of Salta, National Council of Scientific and Technical Research), Av. Bolivia 5150, 4400 Salta, Argentina

Article history
:

Received May 21, 2013
Accepted March 4, 2014

Key words
:

glycosidases, β-glucosidase inhibition and stability in wine, wine aroma, membrane reactor

Summary:

Enzyme activity and stability in a membrane reactor for wine aroma enhancement can be higher than when the enzyme is present in a free state since the catalyst would only be in contact with the low molecular mass components of this beverage. To test this hypothesis, the activity and stability of two commercial β-glucosidases were measured in the presence of Tannat wine and of its low molecular mass fraction (<10 kDa) obtained by ultrafiltration. The relative activities of Endozym Rouge and Endozym β-split β-glucosidases were higher in this fraction (3.8 and 7.6 %, respectively) than in the whole wine (0.9 and 5.6 %, respectively). Both enzymes were also more stable in the low molecular mass fraction. Endozym β-split β-glucosidase retained about 75 % of its initial activity after 14 days in the low molecular mass fraction, as contrasted with only 37.5 % in the wine. The ability of Endozym Rouge β-glucosidase to hydrolyze the synthetic substrate p-nitrophenylglucoside was examined in a simple batch membrane reactor. A rate of hydrolysis comparable to that obtained with the free Endozym Rouge β-glucosidase was reached. Finally, Endozym β-split β-glucosidase was used to hydrolyze the synthetic substrate in a hollow fiber membrane reactor and a substrate conversion near 58 % was achieved.


*Corresponding author:        agcid@unsa.edu.ar
                                                    
    
+54 387 425 1006
                                            +54 387 425 1006

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