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  NLM-PubMed-Logo  doi: 10.17113/ftb.54.01.16.4135

Ascorbate Oxidase-Based Amperometric Biosensor for L-Ascorbic Acid Determination in Beverages


Gábor Csiffáry1*, Péter Fűtő1, Nóra Adányi2 and Attila Kiss2


1Eszterházy Károly College, EGERFOOD Knowledge Centre, Leányka u. 6, HU-3300 Eger, Hungary
2National Agricultural Research and Innovation Centre, Food Science Research Institute,
Herman Ottó út 15, HU-1022 Budapest, Hungary



Article history:

Received February 18, 2015
Accepted September 10, 2015
cc



Key words:
biosensor, l-ascorbic acid, ascorbate oxidase, screen-printed carbon electrodes



Summary:
A novel biosensor for l-ascorbic acid determination in diff erent beverages was elaborated. The ascorbate oxidase enzyme (AAO) from Cucurbita sp., EC 1.10.3.3, was immobilized on a screen-printed carbon electrode with poly(ethylene glycol) (400) diglycidyl ether (PEGDGE) as a crosslinking agent. The standards and samples were measured first with a blank electrode. An inert protein, bovine serum albumin (BSA), was immobilized on the surface of this electrode with PEGDGE. The BSA mass was equivalent to the mass of 10 U of AAO enzyme immobilized on the electrodes (0.021 mg). The linear measuring range for l-ascorbic acid was between 5 and 150 μmol/L. As l-ascorbic acid is a vital vitamin and a common antioxidant used in food industry, fruit juices and vitamin C effervescent tablets were examined. The results were compared to HPLC measurements.






*Corresponding author:  email3  csiffary.gabor@gmail.com
                                      tel3  +36 36 520 400/4186
                                      fax2  +36 36 523 484







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