getpdf

Production of a Fermented Solid Containing Lipases of Rhizopus microsporus and Its Application in the Pre-Hydrolysis of a High-Fat Dairy Wastewater

Dayane Alberton1, David Alexander Mitchell1, Jesús Cordova2,3, Patrício Peralta-Zamora3 and Nadia Krieger3*


1Department of Biochemistry and Molecular Biology, Federal University of Paraná, Centro Politécnico, P.O. Box 19046, Curitiba 81531-980, Paraná, Brazil

2Department of Chemical Engineering, CUCEI, University of Guadalajara, Blvd. Marcelino García Barragán y Calz. Olímpica, 44840 Guadalajara, Jalisco, Mexico
3Department of Chemistry, Federal University of Paraná, Centro Politécnico, P.O. Box 19081, Curitiba 81531-980, Paraná, Brazil

Article history:

Received August 21, 2009
Accepted November 10, 2009

Key words:

lipolysis, enzymatic pretreatment, solid-state fermentation, solid-state cultivation, dairy wastewater, Rhizopus microsporus

Summary:

The filamentous fungus Rhizopus microsporus CPQBA 312-07 DRM was grown in solid-state cultivation and the fermented solid produced was used to hydrolyze triacylglycerols in a high-fat dairy wastewater. For the solid-state cultivation, a mixture of sunflower seed meal and sugarcane bagasse (1:3 by mass on dry basis) was selected. After 18 h of culture, the fermented product had an activity, measured titrimetrically against triolein, of 26 U per gram of dry solids. This substrate mixture does not suffer from compaction and therefore can be used in large scale solid-state cultivation bioreactors. When used to pretreat a high-fat dairy wastewater, with an oil and grease level above 1300 mg/L, the fermented solid reduced the oil and grease level to below 300 mg/L after 72 h at 35 °C. Further work is required to improve the production of lipolytic activity in the solid-state cultivation step and to find the optimum pretreatment time in the wastewater pretreatment step.



*Corresponding author:           nkrieger@ufpr.br
                                               ++55 41 33 613 470
                                               ++55 41 33 613 006

Search FTB


Follow us


 facebook 1 twitter bird_icon LI In Bug

 

QR Code


qrcode

We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information