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Effects of Various Process Parameters on the Production of γ-Linolenic Acid in Submerged Fermentation

Syed U. Ahmed1, Sudheer K. Singh1, Ashok Pandey1*, Sanjit Kanjilal2 and Rachapudi B. N. Prasad2


1
Biotechnology Division, Regional Research Laboratory (Council of Scientific and Industrial Research), Industrial Estate PO, Pappanamcode, Trivandrum 695019, India

2Oils and Fats Division, Indian Institute of Chemical Technology, Hyderabad 500007, India

Article history:

Received November 30, 2005
Accepted March 22, 2006

Key words:

γ-linolenic acid, submerged fermentation, Mucor sp., process parameters

Summary:

Studies were carried out on the fermentative production of γ-linolenic acid (GLA) using seven strains belonging to Mucorales. An oleaginous fungal strain, isolated from the Western Ghats of Kerala produced GLA at a level of 8 % (by mass), when grown in a complex medium containing glucose as the sole carbon source. Effects of different culture conditions were investigated in shake flasks. Maximum dry biomass and total GLA obtained were 48.4 g/L and 636 mg/L, respectively, in the culture cultivated at 30 °C and 200 rpm for 7 days. Among the organic nitrogen sources investigated, yeast extract, and combination of corn steep liquor and baker’s yeast in 1:1 ratio were useful for enhancing the GLA production and the effects were comparable.



*Corresponding author:           pandey@csrrltrd.ren.nic.inashokpandey56@yahoo.co.in
                                               ++91 471 25 15 279
                                               ++91 471 24 91 712

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