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Caprine αs1-Casein Polymorphism: Characterisation of A, B, E and F Variants by Means of Various Biochemical and Molecular Techniques

Maria Feligini1*, Silvia Frati1, Vlatka Cubric Curik2, Ada Brambilla1, Pietro Parma1, Ino Curik3, Gian Franco Greppi1 and Giuseppe Enne1


1
Istituto Sperimentale Italiano »Lazzaro Spallanzani«, Viale Papa Giovanni XXIII 7, I-26900 Lodi, Italy

2Dairy Science Department, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, HR-10 000 Zagreb, Croatia
3Animal Science Department, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, HR-10 000 Zagreb, Croatia

Article history:

Received November 16, 2004
Accepted February 28, 2005

Key words:

Capra hircus, αs1-casein, electrophoresis, RP-HPLC, real-time PCR

Summary:

Considering a wide interest for the characterisation of caprine αs1-casein variants and a large number of differently equipped laboratories, the objective of this study was to analyse and compare characteristics of caprine αs1-casein variants by means of various biochemical and molecular techniques. The most frequent caprine αs1-casein variants (A, B, E and F) were characterized by employing electrophoretic protein separation analyses (capillary zone electrophoresis, isoelectric focusing, and sodium dodecylsulfate polyacrylamide gel electrophoresis), chromatographic analysis (reversed phase-high performance liquid chromatography) as well as DNA analyses (ASA and real-time polymerase chain reaction approach). Further, we stressed weak and strong points for each method applied and provided information for the optimal and complementary use of those methods with respect to time, resolution and costs.


*Corresponding author:           maria.feligini@isils.it
                                               ++39 0371 35 118
                                               ++39 0371 437 070

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