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Optimisation of Inulinase Production by Aspergillus niger Using Simplex and Classical Method

Marcin Skowronek and Jan Fiedurek*


Department of Industrial Microbiology, Maria Curie-Skłodowska University, Akademicka 19, PL-20-033 Lublin, Poland

Article history:

Received March 4, 2004
Accepted July 6, 2004

Key words:

simplex optimisation, inulinase, Aspergillus niger

Summary:

The simplex method has been used to optimise the inulinase production by Aspergillus niger strain 13/36 with respect to 5 components of the medium. The extracellular enzyme activity was almost 80 U/mL, and over 1.6-fold higher than the results obtained by the classical »one-factor-at-a-time« method. The greatest advantage of the simplex method is that the optimum factor levels were determined in only 12 iterative steps, shortening a typical optimum search process considerably. This is the first use of the simplex method for optimising inulinase production.



*Corresponding author:           fiedu@biotop.umcs.lublin.pl
                                               ++48 81 53 75 959

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