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Production of Lactase by Trichoderma sp.

Isil Seyis* and Nilufer Aksoz


Hacettepe University, Faculty of Science, Department of Biology, Beytepe, TR-06532 Ankara, Turkey

Article history:

Received: September 22, 2003
Accepted: April 15, 2004

Key words:

lactase, Trichoderma, pH stability, temperature stability, fungus

Summary:

In order to find an alternative fungal source, 13 different fungi (Aspergillus, Trichoderma, Penicillium, Rhizopus and Fusarium sp.) were cultured in lactase production medium at 30 °C and 150 rpm for 6 days. Experimental results showed that Trichoderma viride ATCC 32098 has maximum lactase specific activity, followed by Trichoderma harzianum 1073 D3. In addition, the studies of stability were carried out in the pH range of 3.0–7.5 at the temperature between 20 and 70 °C. It was observed that the activity of lactase produced from T. viride ATCC 32098 was above 90 % in the pH range of 3.0–7.5 at the temperature between 20 and 60 °C, and even 66 % at 70 °C. It was concluded that Trichoderma sp., especially T. viride ATCC 32098, could be used as an alternative for the production of lactase in industrial scale.



*Corresponding author:           iseyis@hacettepe.edu.tr
                                               ++90 312 29 92 028

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