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Guaiacol Peroxidases in Carrot (Daucus carota L.) Root

Hrvoje Lepeduš1*, Vera Cesar1 and Marijana Krsnik-Rasol2


1
Department of Biology, Faculty of Education, University of J. J. Strossmayer, L. Jägera 9, HR-31000 Osijek, Croatia

2Department of Molecular Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, HR-10000 Zagreb, Croatia

Article history:

Received: October 14, 2003
Accepted: February 16, 2004

Key words:

Daucus carota, guaiacol peroxidases, root

Summary:

Carrot (Daucus carota L.) belongs to the group of common edible vegetables. The aim of the present study was to establish the distribution of guaiacol peroxidases in carrot root sections as well as to determine the enzymatic activity and the electrophoretic pattern of isoperoxidases in tissue extracts of particular root regions: central cylinder, inner cortex and epidermis, including the peripheral region of the cortex. This is a preliminary study for further investigations of stress influence on the expression of peroxidases in carrot root. In the central cylinder and inner cortex region peroxidase activity was localised in tracheary elements. The most intensive enzyme-linked colouration was noticed in the epidermal region of the cortex. The lowest activity was measured in the extracts of primary xylem area, while the area containing inner cortex, secondary xylem and phloem had an almost double activity. The highest activity was measured in the epidermal region. Electrophoretic  analysis of anionic peroxidases revealed one isoenzyme in the central cylinder, and two other isoenzymes in the epidermal region. 



*Corresponding author:           hlepedus@yahoo.com
                                               ++385 31 211 400
                                               ++385 31 212 514

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