getpdf

Improvement of Cell Wall Degrading Enzymes Production by Alginate Encapsulated Trichoderma spp.

Momein H. El-Katatny, Ahmed M. Hetta, Gehan M.Shaban and Hesham M. El-Komy*


Department of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, Egypt


Article history:

Received: February 26, 2003
Accepted: June 9, 2003

Key words:

Trichoderma spp., biocontrol, chitinase, glucanase, immobilization, Ca-alginate

Summary:

Conidia of three Trichoderma isolates were formulated to make alginate pellets with or without 0.5 % chitin or dried fungal mycelium of Fusarium oxysporum as carbon source. The formulations were compared for their ability of in vitro chitinase and β-1,3-glucanase production with free fungal spore suspensions. Conidia entrapped in alginate with or without adjuvant showed high production of enzymes (especially for chitinase) even when repeated 4 times. The addition of chitin or dried fungal mycelium as adjuvant enhanced the enzyme production up to 5 and 2-fold for chitinase and β-1,3-glucanase, respectively. Alginate concentration and surface area of the beads affected the enzyme production. The optimum initial pH, incubation time and temperature were pH=6, 12 days and 40 °C for chitinase, and pH=7, 10 days and 35 °C for β-1,3-glucanase production. The improvement of cell wall degrading enzyme production by alginate encapsulated Trichoderma could explain the in vivo inhibitory effect of such formulations on the target phytopathogenic fungi.



*Corresponding author:           melkatatny@hotmail.com
                                               002 086 344 835
                                               002 086 363 011

Search FTB


Follow us


 facebook 1 twitter bird_icon LI In Bug

 

QR Code


qrcode

We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information