getpdf

Production of Antibodies in Chickens

Mojca Narat


Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia


Article history:

Received: March 13, 2003
Accepted: June 9, 2003

Key words:

antibodies, chicken egg yolk, chicken IgY, monoclonal antibodies, recombinant antibodies, phage display, antibody library, transgenic chickens, immunoglobulin transport

Summary:

Chickens, as a source of desired antibodies, represent an alternate animal system that offers some advantages with respect to animal care, high productivity and special suitability of avian antibodies for certain diagnostic purposes. Despite being an excellent counterpart to mammal IgG chicken IgY antibodies still represent an underused resource. This may be due to the lack of information concerning the possibility of production and application of IgY or their use is being hampered by problems with keeping the chickens and with IgY isolation. As a suggestion how to overcome IgY isolation problems a new immunoaffinity isolation method is presented here. The main purpose of the present work is to provide information on developments and possibilities in the production of chicken IgY. Polyclonal, monoclonal and recombinant forms of IgY, successfully produced so far, as well as their applications are summarised. This article should be a contribution to the efforts of the European Centre for the Validation of Alternative Methods (ECVAM), whose main goal is to promote the scientific and regulatory acceptance of alternative methods, which are of importance to the bioscience and which reduce, refine or replace the use of laboratory animals. 



*Corresponding author:           mojca.narat@bfro.uni-lj.si
                                               ++386 1 7217 916
                                               ++386 1 7241 005

Search FTB


Follow us


 facebook 1 twitter bird_icon LI In Bug

 

QR Code


qrcode

We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information