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Effect of Nitrogen and Carbon Sources on Lipase Production by Penicillium aurantiogriseum

Valéria M. G. Lima1,2, Nadia Krieger1*, Maria Inez M. Sarquis3, David A. Mitchell4, Luiz P. Ramos1 and José D. Fontana2


1
Applied Chemistry Research Centre (CEPESQ), Chemistry Department, Federal University of Parana (UFPR), PO Box 19081, Centro Politécnico, Jardim das Américas, Curitiba 81531-990, Parana, Brazil

2 Biomass Chemo/Biotechnology Laboratory (LQBB), Biochemistry and Molecular Biology Department, UFPR, Curitiba, Parana, Brazil
3FIOCRUZ – Fungal Culture Collection, IOC, Av. Brasil, 4365, Manguinhos, CEP 21045-900, Rio de Janeiro, Brasil
4Solid State Fermentation Laboratory, Biochemistry and Molecular Biology Department, UFPR, Curitiba, Parana, Brazil

Article history:

Received: December 17, 2002
Accepted: April 24, 2003

Key words:

lipases, Penicillium aurantiogriseum, media optimization, lipolytic activity, inorganic nitrogen sources

Summary:

A wild fungal strain isolated from soybean oil and identified as Penicillium aurantiogriseum initially presented a volumetric lipase activity of 0.4 U/mL in submerged culture in a medium containing 0.5 % yeast extract and 1 % olive oil. Studies were undertaken to improve lipase production. The effect of nitrogen source was studied by adding casein peptone, meat peptone, yeast extract or ammonium sulfate to a medium containing potassium nitrate and other mineral salts. The best yield, of 13 U/mL after 72 h, was obtained with the medium supplemented with ammonium sulfate. With the ammonium sulfate concentration increased to double the C/N ratio from 2.5 to 5, a lipolytic activity of 18 U/mL was obtained. Olive, corn, soy and sunflower oils were tested as carbon sources in this medium, with olive oil at 1 % giving a lipolytic activity of 25 U/mL after 48 h, the highest yield obtained in this study. Enzyme production was best at 29 °C, within a range tested from 26 to 32 °C. These results are promising because this strain produces lipase in an inexpensive inorganic medium and we succeeded in increasing the lipolytic activity 62-fold over the initial values obtained with the non-optimized medium. 



*Corresponding author:    nadiak@química.ufpr.br

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