getpdf

Curcumin – A Potent Inhibitor of Galectin-3 Expression

Jerka Dumić*, Sanja Dabelić and Mirna Flögel


Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, HR-10000 Zagreb, Croatia


Article history:

Received: October 3, 2002
Accepted: November 7, 2002

Key words:

galectin-3, curcumin, UV light, alkylating agent MNNG

Summary:

The expression of galectin-3, a β-galactoside binding lectin, was found to be affected by different kinds of stressors, and is strongly modified in numerous physiological and pathophysiological conditions. Although no precise regulatory mechanisms of galectin-3 expression are unraveled, transcription factors AP-1 (activator protein 1) and NF-κB (nuclear factor kappa B) play an important role in these processes. Activities of both transcription factors are affected by curcumin, a biologically active compound extracted from rhizomes of Curcuma species. We have analyzed the impact of curcumin on the expression of galectin-3 in glioblastoma cells under basal conditions and under stress invoked by the cell exposure to alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and ultraviolet C (UV-C) light. Galectin-3 level was measured by western-blot technique using M3/38 monoclonal antibody. Curcumin has decreased the basal level of galectin-3, while the pretreatment of cells with curcumin has considerably reduced the inducible effect of UV-C radiation and abolished the inducible effect of alkylating agent. Thus, curcumin has been identified as a potent inhibitor of galectin-3 expression. 



*Corresponding author:           jdumic@pharma.hr
                                               +385 1 4818 757
                                               +385 1 4856 201

Search FTB


Follow us


 facebook 1 twitter bird_icon

 

QR Code


qrcode

We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information