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Development of a New Colorimetric Chemosensor for Selective Determination of Urinary and Vegetable Oxalate Concentration Through an Indicator Displacement Assay (IDA) in Aqueous Media

 


Hossein Tavallali*orcid tiny, Gohar Deilamy-Radorcid tiny and Narges Mosallanejadorcid tiny

 

 

 

Department of Chemistry, Payame Noor University, P.O. Box 19395-3697, Tehran, Iran

 

 


Article history:
Received: 14 February 2018
Accepted: 8 May 2018

 

 


Key words:
colorimetric chemosensor, indicator displacement assay, copper complex, oxalate, urine

 

 


Summary:
The paper proposes a method that exhibits operational simplicity for the indirect spectrophotometric determination of oxalate ion. We developed Reactive Blue 4 (RB4) as a sensor by complexation with copper ion as a simple, inexpensive yet selective colorimetric chemosensing ensemble for the recognition of oxalate over other available competitive analytes via indicator displacement assay (IDA) in both solution (aqueous medium) and solid state (paper-based experiment). The addition of oxalate to RB4-Cu2+ complex changed the colour from sky blue to dark blue due to the regeneration of RB4 by the chelation of oxalate as the competitive analyte with Cu2+. The absorbance band increase is linear with oxalate concentration from 1.76 to 49.4 µmol/L with a detection limit of 0.62 µmol/L. This measurement mode did not show any influence of interferences (available anions and ascorbic acid). This approach eliminated the need for the separation stages, enzymatic multiple-step reactions, sample preparation, organic solvent mixture, chemical modifications and equipment developed to a high degree of complexity. The oxalate determination gave results in different real samples such as urine, mushroom and spinach, which demonstrated the applicability of the existing method. Furthermore, this colorimetric system can serve as IMPLICATION molecular logic gate using Cu2+ and oxalate (C2O2–) as inputs and UV-Vis absorbance signal as the output with potential monitoring applications.

 

 


*Corresponding author: tel3 +989173153520
                                          fax2 +987136222249
                                          email3  Tavallali@pnu.ac.ir

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