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Evaluation of the Antioxidant Capacity, Antimicrobial and Antiproliferative Potential of Fir (Abies alba Mill.) Honeydew Honey Collected from Gorski kotar (Croatia)


Dalibor Broznić1*orcid tiny, Ivana Ratkaj2orcid tiny, Mladenka Malenica Staver2orcid tiny, Sandra Kraljević Pavelić2,3orcid tinyPaula Žurga4orcid tiny, Dragan Bubalo5orcid tiny and Ivana Gobin6orcid tiny


1Department of Chemistry and Biochemistry, Faculty of Medicine, University of Rijeka, Braće Branchetta 20, HR-51000 Rijeka,
  Croatia

2Department of Biotechnology, University of Rijeka, Radmile Matejčić 2, HR-51000 Rijeka, Croatia
3Department of Biotechnology, Centre for High Throughput Technologies, University of Rijeka, Radmile Matejčić 2, HR-51000
  Rijeka, Croatia

4Teaching Institute of Public Health of Primorsko-goranska County, Krešimirova 52a, HR-51000 Rijeka, Croatia
5Faculty of Agriculture, University of Zagreb, Svetošimunska 25, HR-10000 Zagreb, Croatia
6Department of Microbiology, Faculty of Medicine, University of Rijeka, Braće Branchetta 20, HR-51000 Rijeka, Croatia


Article history:
Received: 14 January 2018
Accepted: 27 July 2018



Key words:
fir (Abies alba Mill.) honeydew honey, cell cycle, apoptosis, antimicrobial activity, antioxidant capacity, kinetic analysis


Summary:
The paper examines the antiproliferative, antimicrobial and antioxidative effects of fir (Abies alba Mill.) honeydew honey from mountain region of Croatia (Gorski kotar) as a potential replacement for standard antibiotics and chemotherapeutic agents. Cell viability, annexin V assay and flow cytometry analysis served to analyse the antiproliferative effect on, apoptosis induction in and cell death of cancer cell lines: HeLa, MCF-7, SW620, CFPAC-1, MIA PaCa-2 and normal diploid human fibroblasts (BJ). Antimicrobial activity was tested against Staphylococcus and Acinetobacter strains by agar well diffusion and microdilution assays. The DPPH˙ assay determined the radical scavenging activity, while mathematical models helped to evaluate the kinetic data of DPPH˙ inhibition. Antiproliferative effect on all tested cell lines and the prominent effect on normal diploid human fibroblasts (BJ), colorectal adenocarcinoma (SW620, metastatic) and breast epithelial adenocarcinoma (MCF-7, metastatic) was observable. The mechanisms of antiproliferative effect included accumulation of cells in the sub-G1 phase in all tested cells and induction of apoptosis in SW620 and MCF-7 cells predominantly. The antibacterial assays showed that antibiotic-resistant strains of both bacteria, including multi-resistant strain A. baumannii ATCC® BAA1605™, were sensitive to all tested honey samples. Radical scavenging assay suggests that antioxidants present in the honey possess different radical suppressing abilities and that they react at different rates with radicals, thereby causing two steps of reaction. The results of the study indicate that Croatian fir honeydew honey has a therapeutic potential due to the strong biological activity and can serve to protect human health.



*Corresponding author:  tel3  +38551651132
                                           fax2  +38551678895
                                            email3  dalibor.broznic@medri.uniri.hr

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