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Effect of Tween 80 and Acetone on the Secretion, Structure and Antioxidant Activities of Exopolysaccharides from Lentinus tigrinus


Peixin He1,2, Shuangshuang Wu1, Lige Pan1, Siwen Sun1, Duobin Mao1,2 and Chunping Xu1,2*


1School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China
2Collaborative Innovation Center of Food Production and Safety, Henan Province, Zhengzhou 450001, PR China




Article history:
Received April 14, 2015
Accepted April 14, 2016

 

Key words:
exopolysaccharides, Lentinus tigrinus, molecular mass, molecular conformation, antiproliferative activity

 

Summary:
In this study, the effects of the addition of Tween 80 and acetone on secretion, structure and antioxidant activities of Lentinus tigrinus exopolysaccharides (EPS) were investigated. It was found that Tween 80 and acetone displayed a stimulatory effect on EPS secretion. The EPS obtained by the addition of Tween 80 (EPS-T), acetone (EPS-A) and control (EPS-C) were purified by Sepharose CL-6B gel filtration chromatography and molecular mass of purified fractions was estimated to be 22.1, 137 and 12 kDa, respectively. Monosaccharide composition analysis indicated that EPS-T, EPS-A and EPS-C were mainly composed of glucose and mannose. Congo Red test indicated that EPS-T and EPS-A had a highly ordered conformation of triple helix, while EPS-C had a random coil conformation. Furthermore, EPS-A exhibited higher DPPH scavenging and antiproliferative activities than EPS-C and EPS-T, which might be attributed to the molecular mass.




 


*Corresponding author:  email3   c.p.xu@zzuli.edu.cn
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