Differentiation of Commercial PDO Wines Produced in Istria 
(Croatia) According to Variety and Harvest Year Based on 
HS-SPME-GC/MS Volatile Aroma Compound Profiling

Igor Lukić* and Ivana Horvat

Institute of Agriculture and Tourism, Karla Huguesa 8, HR-52440 Poreč, Croatia

Article history:
Received  June 30, 2016
Accepted  November 11, 2016


Key words:
monovarietal wine, Croatia, volatile aroma compounds, variety, harvest year, HS-SPME-GC/MS


To differentiate monovarietal wines made from native and introduced varieties in Istria (Croatia), samples of Malvazija istarska, Chardonnay and Muscat yellow from two harvest years (2013 and 2014) were subjected to headspace solid-phase microextraction and gas chromatographic/mass spectrometric analysis (HS-SPME-GC/MS) of volatile aroma compounds. Significant effects of variety and harvest year were determined, but their interaction complicated the differentiation. Particular compounds were consistent as markers of variety in both years: nerol for Malvazija, ethyl cinnamate and a tentatively identified isomer of dimethylbenzaldehyde for Chardonnay, and terpenes for Muscat yellow. Wines from 2013 contained higher concentrations of the majority of important volatiles. A 100 % correct differentiation of Malvazija istarska and Chardonnay wines according to both variety and harvest year was achieved by stepwise linear discriminant analysis.


*Corresponding author:  email3
                                      tel3  +385 (0)52 408 327
                                      fax2  +385 (0)52 431 659