https://doi.org/10.17113/ftb.57.02.19.5832       


Immobilization of Horseradish Peroxidase on Multi-Armed Magnetic Graphene Oxide Composite: Improvement of Loading Amount and Catalytic Activity   


Junhui Rong¹, Zijuan Zhou², Yun Wang¹*, Juan Han³, Chunmei Li¹, Wenli Zhang¹ and Liang Ni<sup>1  


1</sup>School of Chemistry and Chemical Engineering, Jiangsu University, 212013 Zhenjiang, PR China 
²School of Food Science and Nutrition, University of Leeds, Leeds, LS2 9JT, UK 
³School of Food and Biological Engineering, Jiangsu University, 212013 Zhenjiang, PR China   



Article history: 
Received: 6 May 2018 
Accepted: 8 May 2019
  



Key words: 
immobilized enzyme, graphene oxide, multi-armed polymer, horseradish peroxidase, synergistic effect    



Summary: 
In this study, a novel type of multi-armed polymer (poyltehylene glycol, PEG) magnetic graphene oxide (GO) composite (GO@Fe₃O₄@6arm-PEG-NH₂) has been synthesized as a support for immobilization of horseradish peroxidase (HRP) for the first time. The loading amount of HRP was relatively high (186.34 mg/g) due to the surface of carrier material containing a large amount of amino groups from 6arm-PEG-NH₂, but degradation rate of phenols was also much higher (95.4 %), which is attributed to the synergistic effect between the free HRP (45.4 %) and the support material of GO@Fe₃O₄@6arm-PEG-NH₂ (13.6 %). Compared with the free enzyme, thermal, storage and operational stability of the immobilized HRP improved. The immobilized HRP still retained over 68.1 % activity after being reused 8 times. These results suggest that the multi-armed magnetic composite has good application prospect for enzyme immobilization.   




*Corresponding author:    +8615896385156 
                                             yunwang@ujs.edu.cn