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Hydroethanolic Extract of Grape Peel from Vitis labrusca Winemaking Waste: Antinociceptive and Anti-Inflammatory Activities

Cristiana F. G. Silva1orcid tiny, Victor Fattori2orcid tiny, Caroline R. Tonetti1orcid tiny, Marcos A. S. Ribeiro3orcid tiny, Ricardo L. N. Matos4orcid tiny, Jéssica B. Carra1orcid tiny, Eduardo C. Meurer3orcid tiny, Elisa Y. Hirooka5orcid tiny, Janice A. Rafael6orcid tiny, Sandra R. Georgetti4orcid tiny, Marcela M. Baracat4orcid tiny, Waldiceu A. Verri Jr2orcid tiny and Nilton S. Arakawa1,4*orcid tiny

1Department of Chemistry, State University of Londrina, Rod. Celso Garcia Cid, Km 380, Campus Universitário, 86057-970, Londrina, PR, Brazil

2Department of Pathology, State University of Londrina, Rod. Celso Garcia Cid, Km 380, Campus Universitário, 86057-970, Londrina, PR, Brazil

3Department of Exact Sciences, Federal University of Paraná, R. Dr. João Maxímiano, 426, Vila Operária, 86900-000 Jandaia do Sul, PR, Brazil

4Department of Pharmaceutical Sciences, State University of Londrina, Av. Robert Koch, 60, Operária, 86038-440, Londrina, PR, Brazil

5Department of Food Science and Technology, State University of Londrina, Rod. Celso Garcia Cid, Km 380, Campus Universitário, 86057-970, Londrina, PR, Brazil

6Pharmaceutical Science Course, Filadelfia University Center, Av. Juscelino Kubitschek, 1626, Centro, 86020-000, Londrina, PR, Brazil

Article history:

Received: 30 November 2020

Accepted: 12 November 2021

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Key words:

agro-industrial residue; analgesia; anthocyanins; phenolic compounds


Research background. Extracts from grape pomace, including the wine, showed many biological effects such as antioxidant and anti-inflammatory activities. Unfortunately winemakers discard the bagasse and the waste is less useful, however it contains bioactive compounds which result in antioxidant and anti-inflammatory properties. The work aimed to analyze the hydroethanolic extract of peels from agro-industrial waste from Vitis labrusca and to evaluate its antinociceptive and anti-inflammatory assays. This study is relevant for reusing a residue and adding value to the grape economic chain. 

Experimental approach. A representative sample of pomace was obtained and the peels were applied to produce the extract. The phenolic compounds were determined by multiple reaction monitoring mode of mass spectrometry methods and Folin-Ciocalteu, using gallic acid as standard. The biological analyzes were carried out using mice orally treated with crude extract at doses (30, 100 and 300 mg/kg). We evaluated mechanical hyperalgesia by the von Frey method, thermal heat hyperalgesia using a hot plate at 55 °C, paw edema using a pachymeter, and neutrophil recruitment by measurement of myeloperoxidase enzyme activity. The nephrotoxicity and hepatotoxicity were evaluated by biochemical analyses using blood samples that were collected after the Vitis labrusca administration.

Results and conclusions. The peels correspond to 75 % of all wet winemaking residue and 59 % on a dry basis. We identified nine anthocyanins (3-O-glucoside: peonidin, delphinidin, petunidin, and malvidin; 3-p-coumaroyl-glucoside: cyanidin, peonidin, petunidin, and malvidin, and malvidin-3,5-diglucoside), five flavonoids (apigenin-7-glucoside, luteolin-7-glucoside, quercetin-3-galactoside, isorhamnetin-3-glucoside, and myricetin-3-rutinoside), and 26.62 mg GAE/g of phenolic compounds. In vivo assays, showed that Vitis labrusca extract at concentrations 100 and 300 mg/kg reduced carrageenan-induced mechanical and thermal hyperalgesia, 50 % of the paw edema, and neutrophil recruitment. In addition, there were no nephrotoxicity and hepatotoxicity. Our extract obtained from winemaking residue has analgesic and anti-inflammatory action, related at least in part to the presence of phenolic compounds, and it has no toxicity to renal and hepatic tissues.

Novelty and scientific contribution. We demonstrated that this waste can be used for the production of antioxidant and anti-inflammatory products (pharmaceutical and cosmetics) without toxicity, contributing to the environmental economy.

*Corresponding author: +55(43)33712475

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