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Isolation and Quantification of Triterpenoid Acids from Ganoderma applanatum of Istrian Origin

Bojana Boh1, Damjan Hodžar1*, Danica Dolničar1, Marin Berovič2 and Franc Pohleven3


1
Faculty of Science and Engineering, University of Ljubljana, Vegova 4, P.O.B. 18/1, 1000 Ljubljana, Slovenia

2National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
3Biotechnical Faculty, University of Ljubljana, Rožna dolina, c. VIII 34, 1000 Ljubljana, Slovenia

Article history:

Received October 21, 1999
Accepted January 21, 2000

Key words:

Ganoderma applanatum, triterpenoids, ganoderic acids, ganoderenic acids, extraction, isolation, quantification

Summary:

Various Ganoderma spp. have been known for their wide range of pharmacologically active compounds. From Ganoderma applanatum of Istrian origin, growing on Populus, triterpenoid acids were isolated and quantified. An in-house relational information system was built and used as a support for isolation and identification of triterpenoids from different layers of G. applanatum fruiting bodies. An optimised extraction procedure was developed, and IR, UV and NMR spectra were used for the identification of ganoderic and ganoderenic acids. The highest fraction of triterpenoid acids was found in the tubes (6.4 mg/g of air-dry weight), followed by the dark context layer, which is the young part of the pileus (2.5 mg/g). The white context layer of the older pileus and the upper surface of the fruiting body contained only about 0.6 mg/g of triterpenoid acids. 



*Corresponding author:           bojana.boh@uni-lj.si
                                               ++386 (0)61 214 326
                                               ++386 (0)61 1258 684

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