Construction of a cDNA-based K1/K2IK28 Triple Killer Strain of Saccharomyces cerevisiae

Manfred J. Schmitt*, Gerhard Schernikau1

Angewandte Molekularbiologie der Universität des Saarlandes, FR 13.3, Gebaude 2, 0-66041 Saarbrücken, Germany
Institut für Mikrobiologie end Weinforschung, Johannes Gutenberg-Universität Mainz, D-55099 Mainz, Germany

Article history:

Received September 10, 1997
Accepted November 24, 1997

Key words:

S. cerevisiae, killer toxin expression vectors, triple killers


By transforming a natural, dsRNA-based K1 killer strain of the wine yeast Saccharomyces cerevisiae with two multi-copy (2µ) vectors carrying cDNA copies of the K2 and K28 preprotoxin/immunity genes, a triple killer strain has been constructed that (i) simultaneously secreted three different killer toxins [Kl, K2, K28], (ii) expressed functional toxin immunities, and (Hi) exhibited a strong and significantly broader killing spectrum than the single killer derivatives. Both plasmids were shown to be self-selective under conditions where the triple killer was cultivated in YEPD medium at pH - 4.7. It is proposed that recombinant K1/K2/K28 triple killers should be able to predominate in mixed yeast cultures and therefore might be useful in industrial wine fermentations to prevent stuck fermentations and/or killer yeast contaminations.



*Corresponding author:  
    (+49) 681 302 4730
                                                (+49) 681 302 4710

Search FTB

Follow us

 facebook 1 twitter bird_icon LI In Bug


Environmental Policy

sdg publishers compact 4 300x300

QR Code


We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information