Transformation of Different Dyes by Extracellular Ligninases from Phanerochaete chrysosporium
Helena Podgornik, Lucija Bradeško and A. Perdih
Faculty of Chemistry and Chemical Technology, University of Ljubljana, P.O.B. 337, 61000 Ljubljana, Slovenia
Received July 11, 1994
Accepted June 19, 1995
More then 50 structurally different dyes were tested for dccolorizntion by extracellular ligninases from Phanerochaete chrysosporium. They can be divided into four main groups according to the type of dccolorizntion. The influence of dyes structure on dccolorizntion was not observed. The lime course of spectral clianges was followed spcclrophotomctrically and kinetic curves at specific wavelength were taken after that. The rate of decolorizalion for majority of dyes could be calculated. This value indicated which dyes could be used for ligninolytic activity measurements and how fast they could be biodegraded by ligninases.
Presented at the 2nd Croatian Congress of Food Technologists, Biotechnologists and Nutritionists„ Zagreb, June1.5-17, 1994