Biosorption and Biodegradation of Humic Substances by Trichoderma viride

Mira Peirović1, Felicita Briški2, Marija Kaštelan-Macan1

Laboratory of Analytical Chemistry,

2Laboratory of Industrial Ecology, Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 41000 Zagreb

Article history:

Received September 13, 1993

Accepted October 21, 1993


Mycelial pellets of Trichoderma viride were used for biodegradation of naturally occurring and commercial humic substances from aqueous solutions. Experiments were carried out at 25 °C and pH = 6.0. Removal of humic substances from the aqueous phase was monitored by following the COD-elimination, fungal growth and decrease of absorbance at 370 nm. Within 10 days 42 % of HAS (soil humic acids), 44 % of FAS (soil fulvic acids) and 67 % of HAF (Fluka humic acids) were removed from the solution. It was found thai beside degradation, adsorption of humic substances onto fungal mycelia play a significant role in this process.

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