getpdf     https://doi.org/10.17113/ftb.53.01.15.3661

Potential of Different Coleus blumei Tissues for Rosmarinic Acid Production

Nataša Bauer1§*, Rosemary Vuković, Saša Likić3 and Sibila Jelaska1

1Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 
HR-10000 Zagreb, Croatia
2Department of Biology, University of Osijek, Cara Hadrij ana 8/A, HR-31000 Osijek, Croatia
3
Department of Botany, Faculty of Science, University of Zagreb, Marulićev trg 9a, HR-10000 Zagreb, Croatia

Article history:
Received March 31, 2014
Accepted January 14, 2015


Key words:
abscisic acid, bioreactor, callus, Coleus blumei, 2,4-dichlorophenoxyacetic acid, 
hairy root, 1-naphthaleneacetic acid,
rosmarinic acid, root, tumour

 

Summary:
Rosmarinic acid is one of the main active components of Coleus blumei and is known to have numerous health benefits. The pharmacological significance of rosmarinic acid and its production through in vitro culture has been the subject of numerous studies. Here, the ability of different tissues to accumulate rosmarinic acid and sustainability in production over long cultivation have been tested. Calli, tumours, normal roots and hairy roots were established routinely by application of plant growth regulators or by transformation with agrobacteria. The differences among the established tumour lines were highly heterogeneous. Hairy root lines showed the highest mean growth rate and consistency in rosmarinic acid production. Although some tumour lines produced more rosmarinic acid than the hairy root lines, over a long cultivation period their productivity was unstable and decreased. Further, the effects of plant growth regulators on growth and rosmarinic acid accumulation were tested. 2,4-Dichlorophenoxyacetic acid significantly reduced tumour growth and rosmarinic acid production. 1-Naphthaleneacetic acid strongly stimulated hairy root growth whilst abscisic acid strongly enhanced rosmarinic acid production. Hairy roots cultured in an airlift bioreactor exhibited the highest potential for mass production of rosmarinic acid.


*Corresponding author:     nbauer@zg.biol.pmf.hr  
                                                     +385 1 4606 263
                                         +385 1 4606 286



§These authors contributed equally to this work

 

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