Ultrasound-Assisted Extraction and Biological Activities of Extracts of Brassica oleracea var.
Valéria Dal Prá1, Carolina Bolssoni Dolwitsch1, Fernanda Oliveira Lima2, Camilo Amaro de Carvalho3, Carine Viana1,
Paulo Cícero do Nascimento4 and Marcelo Barcellos da Rosa1,4*
1Post-Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, Camobi Campus, 97105-900 Santa
Maria, RS, Brazil
2Federal University of Fronteira Sul, Centro, 97900-000 Cerro Largo, RS, Brazil
3Medical and Nursing Department, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, 36570-000 Viçosa, Brazil
4Post-Graduate Program in Chemistry, Federal University of Santa Maria, Camobi Campus, 97105-900 Santa Maria, RS, Brazil
Received October 4, 2013
Accepted January 26, 2015
Brassica oleracea var. capitata, ultrasound-assisted extraction, hydrolysis of extracts, biological activity
In this work, the antioxidant and antimicrobial activities of Brassica oleracea var. capitata extracts obtained through ultrasound-assisted extraction are evaluated. The extracts obtained using the best extraction conditions were subjected to different hydrolysis conditions before their use in the biological tests. The crude and hydrolysed extracts were characterized using gas chromatography coupled with a mass detector. The use of ultrasound at 30 °C with 60 % (by volume) solvent enabled obtaining a richer extract. All extracts had antioxidant activities against DPPH (13.0–80.0 %), superoxide (35.2–63.2 %) and peroxyl (89.3–99.5 %) radicals, but the use of hydrolysed extracts considerably improved the antioxidant activities. Antimicrobial activities only of the hydrolysed extracts of Brassica oleracea var. capitata were detected. It was confirmed that antioxidant activity of vegetable extracts can be considerably increased when hydrolysis is applied as a pretreatment to their extraction.
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