Sensitive Voltammetric Determination of Natural Flavonoid Quercetin on a Disposable Graphite Lead

Dai Long Vu1, Simona Žabčíková1, Libor Červenka1*, Bensu Ertek2 and Yusuf Dilgin2

1University of Pardubice, Studentska 573, CZ-53210 Pardubice, Czech Republic
2Canakkale Onsekiz Mart University, Mart University 18, TR-17100 Canakkale, Turkey

Article history:
Received March 18, 2015
Accepted July 8, 2015

Key words
pencil graphite electrode, voltammetry, quercetin, fruit juices

In this paper, a pencil graphite electrode was pretreated using chronoamperometry technique in phosphate buffer solution (pH=7.0) for sensitive determination of quercetin. Oxidation of quercetin was investigated using pretreated pencil graphite electrode and anodic stripping differential pulse voltammetry. Under optimal conditions, the anodic current of quercetin exhibited linear response to its concentration in the range from 0.001 to 1.5 μmol/L with the limit of detection of 0.3·10–3 μmol/L. The proposed method was successfully applied for the determination of quercetin in cranberry and blackcurrant juices with recovery rate from 93.2 to 94.7 %. Solid-phase extraction was found to be necessary prior to voltammetric determination of 
quercetin in fruit juice samples using pretreated pencil graphite electrode.


*Corresponding author:   email3
                                       tel3   +420 466 037 718
                                       fax2   +420 466 037 068

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