getpdf  NLM-PubMed-Logo  https://doi.org/10.17113/ftb.56.03.18.5318 

Linseed (Linum usitatissimum) Oil Extraction Using Different Solvents

 

Guilherme Sabadin Piva1orcid tiny, Thiago André Weschenfelder1orcid tinyElton Franceschi2orcid tiny, Rogério Luis Cansian1orcid tiny, Natalia Paroul1orcid tiny and Clarice Steffens1*orcid tiny

 

 


1Department of Food Engineering, URI Erechim, Av. Sete de Setembro 1621, 99700-000, Erechim, RS, Brazil
2Colloidal System Research Center (NUESC), Research and Technology Institute (ITP), Tiradentes University (UNIT), 49032-490, Aracaju, SE, Brazil

 

 


Article history:
Received: 24 April 2017
Accepted: 23 May 2018 
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Key words:
linseed oil, fatty acids, extraction yield, extraction methods, oil quality

 

 


Summary:
This work aims at characterizing linseed oil obtained using different extraction methods (hexane, subcritical propane and pressurized ethanol), and comparing the results with commercial linseed oil extracted by cold mechanical press method. An experimental design helped to evaluate temperature and pressure effects on the oil extraction using propane and ethanol. Gas chromatography assisted in evaluating the essential fatty acids. There were no significant differences among the ω-3, 6 and 9 fatty acids from linseed oil obtained using the different extraction methods. Only the acidity of linseed oil extracted by subcritical propane (0.956 %) showed significant differences among the physicochemical parameters. Extraction using organic solvent (Soxhlet) gave a 36.12 % yield. Extraction using subcritical propane at 107 Pa and 40 °C for 1.5 h gave a better yield (28.39 %) than pressurized ethanol (8.05 %) under similar conditions. Linseed oil extraction using subcritical propane was economically viable, resulting in a 124.58 US$/L product cost. The results present subcritical propane extraction as a promising alternative for obtaining linseed oil at mild temperature and pressure conditions, without losing quality and quantity of fatty acids such as ω-3, 6 and 9.

 

 


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Introduction

Linseed (Linum usitatissimum) oil contains 40–50% α-linolenic (ω-3) acid, which is rich in phytosterols and tocopherols. These bioactive components can improve the human immune system, prevent inflammation, reduce bad cholesterol absorption and help cardiovascular disease prevention (1, 2).

Vegetable oil industrial production uses mechanical press extraction. This method allows the utilization of residual cake, with low initial operation costs, and eliminates contaminants. However, it is not an efficient extraction process, extracting less than 70% oil content from the seed (3). Extraction with organic solvents (hexane, ethanol, heptane and isobutene) in industrial plants yields around 99% oil. However, this process shows a series of disadvantages including time-consuming procedures, low selectivity, solvent contamination and degradation of key bioactive components (3). Pressurized liquid extraction (PLE) with solvents occurs at temperatures above boiling point, when pressure in the extraction system increases. Pressure reduces solvent surface tension, which improves penetration into the matrix pores, resulting in rupture and increase of mass transfer, thus improving extraction efficiency (4-7).

Subcritical fluid extraction (SFE) has some advantages when compared to an organic solvent (n-hexane) extraction, such as its use at low temperatures, short extraction time, good selectivity, and residual solvent elimination (8). The subcritical propane allows high extraction rates in processes with vegetable oil, due to the high triglyceride solubility in this solvent. Furthermore, it may be used at low pressures, unlike supercritical carbon dioxide (scCO2), which is a major advantage for the oil industry (9). Literature offers works related to the use of subcritical propane for pequi (10), sesame seed (11), sunflower (12) and linseed (2) oil extractions.

Extractions at low pressures and temperatures are attractive from an economic standpoint, giving high yield in short extraction time, hence reducing costs (13). In the same work extraction of coriander seed oil using propane as a solvent, under supercritical and subcritical conditions, using 2·107 and 3·107 Pa at 35 °C for CO2 and 5·106, 8·106 and 107 Pa at 25 °C for propane indicated that propane solvation power was much higher than CO2 (13).

Pradhan et al. (3) and Khattab and Zeitoun (14), using supercritical CO2 as a solvent for linseed oil extraction, at 3·107 Pa and 50 °C for 3 h and 4·107 Pa and 50 °C for 2 h obtained 35.3 and 36.5% yield, respectively. Zanqui et al. (2) obtained the highest yield (28.8%) of linseed oil extracted with subcritical propane at 60 °C and 1.2·107 Pa for 5 h.

Due to linseed oil potential, studies to maximize production and improve oil quality by increasing the content of fatty acids are vital. Thus, the aim of this work is to evaluate three different linseed oil extraction methods (SFE, PLE and Soxhlet), in terms of yield, essential fatty acid content and physicochemical quality, in comparison with commercial oil.

MaterialS and Methods

Samples

Brown linseed (Linum usitatissimum) samples (5 kg) were obtained from the 2014–2015 harvest. The commercial linseed samples came from a local market (Pazze Foods Company), cultivated in the Panambi municipality (28°17´33´´ latitude and 53°36´47´´ longitude), Rio Grande do Sul State, Brazil. Mechanically pressed brown linseed oil (150 mL) came from Pazze Foods Company (Panambi, RS, Brazil).

Sample preparation

The linseed samples were oven-dried (MA035/1; Marconi Equipamentos para Laboratórios Ltda., Piracicaba, SP, Brazil) at 50 °C for 48 h, vacuum packed using a Selovac 200B (Selovac, São Paulo, SP, Brazil) and stored in a freezer (Brastemp, São Paulo, SP, Brazil) at –18 °C. In order to perform oil extractions, samples were crushed in a mixer Cuisinart Brasil (São Paulo, SP, Brazil), and sieved through 16 and 32 mesh sizes, using the fraction retained in the 32 mesh sieve after passing the 16 mesh one, obtaining particle sizes of 500 to 1000 µm.

Extraction methods

Organic solvent

The organic solvent extraction (n-hexane, 95% purity, Cinética, Londrina, PR, Brazil) in a Soxhlet equipment (Fisher Scientific, Thermo Fisher Scientific, Pittsburgh, PA, USA) lasted 14 h, at 60–80 °C with approx. 20 g ground linseed powder. After extraction, the sample was concentrated in a rotary evaporator (Q344B; Quimis, São Paulo, SP, Brazil) under vacuum at 40 °C. All extractions were performed in triplicate.

Subcritical propane

The extraction occurred in the packed bed extractor using propane with 99.97% purity (White Martins Gases Industriales Ltda., Rio de Janeiro, RJ, Brazil) in a subcritical state. The extraction equipment consisted of a solvent reservoir, two thermostatic baths (521/3D; Nova Ética, Vargem Grande do Sul, SP, Brazil), one syringe pump (500D; Teledyne ISCO, Lincoln, NE, USA) and a 62.8-mL jacketed extraction vessel. Evaluation conditions were: 20, 40 and 60 °C and 8·106, 107 and 1.2·107 Pa. All extrac-tions required a constant 2 mL/min flow rate. Ground linseed powder (16 g) was placed into the extraction vessel (a heating jacket with a thermostatic bath, model SE 100AG; Servilab, Le Mans, France), supported by two 150 mesh wire disks at both ends. The solvent was pumped at a constant flow rate into the extraction chamber and kept in contact with the bed and samples until reaching the desired extraction pressure. Extraction lasted for 90 min. After that, the solvent feed was interrupted and the valve located at the bottom of the chamber was opened, obtaining the extracted oil mass. The micrometric expansion valve was wrapped in the temperature controller (HW 1440; Coel, São Paulo, SP, Brazil) to keep at a constant 55 °C. Linseed oil yield (%) was calculated using the following equation:
where Y is the yield of linseed oil (in %), m(seed powder) is the seed powder mass and m(extracted oil) is the mass of oil extracted at the process end. All extractions were performed in triplicate.

Pressurized ethanol

For pressurized liquid extraction (PLE) the measuring cylinder containing ethanol (99% purity; Vetec, Rio de Janeiro, RJ, Brazil) was connected to the HPLC pump (Lab Alliance Series III, New York, NY, USA) with two thermostatic baths (521/3D; Nova Ética). Evaluation conditions were: 20, 40 and 60 °C and 8·106, 107 and 1.2·107 Pa. All extractions required a constant 2 mL/min flow rate, using 16 g sample in the extraction cell. During the extraction step, the sample was loaded into the cell and the solvent was added at an operational pressure, until reaching the operational temperature. When the pressure reached the desired value, pump pressure was interrupted, and the system was isolated by closing the valve that releases the liquid to the pump, keeping it pressurized for 15 min. Then, the pump was once again engaged, the micrometric valve was opened starting the extraction. Extraction lasted 90 min, and samples containing oil and ethanol were placed in an oven coupled to a vacuum pump (Quimis) at 50 °C, for solvent evaporation. Yield was calculated using Eq. 1. All extractions were performed in triplicate.

Experimental design

A 22 experimental design with 3 central points using pressure of 8·106, 107 and 1.2·107 Pa and temperature of 20, 40 and 60 °C for 90 min evaluated the effects of temperature and pressure on the extraction yield using subcritical propane and pressurized ethanol.

Physicochemical analysis of linseed oil

Acidity, refraction index, and density of the oil obtained with both extraction methods and the commercial oil were analyzed according to the methods described by the Adolfo Lutz Institute, São Paulo, Brazil (15). All physical and chemical analyses were performed in triplicate. Titratable acidity was determined by titrating a sample with 0.1 M sodium hydroxide solution (NaOH; Synth, Diadema, SP, Brazil) and phenolphthalein as indicator. The refractive index was measured with an Abbé refractometer (Delta, São Paulo, SP, Brazil). Density was determined using a density bottle (4500 density meter; DMA, São Paulo, SP, Brazil) at 25 °C.

Qualitative analysis of essential fatty acids

The samples were esterified following the method set by the Adolfo Lutz Institute (15). A mass of 0.1 g oil was mixed with 0.2 mL (2 M) methanolic potassium hydroxide solution (KOH; Synth, and methanol; Merck, São Paulo, Brazil) and 2 mL n-hexane, and homogenized in a vortex mixer (Q220M; Quimis) for 30 s. After that, 3 mL saturated sodium chloride solution (NaCl; Cinética, Londrina, PR, Brazil) were added and the supernatant was then removed.

Chromatographic analysis

Qualitative analysis of essential fatty acids followed the instructions of Khattab and Zeitoun (14), in a gas chromatograph (CG-2010 Plus model; Shimadzu do Brazil, Barueri, SP, Brazil) equipped with a polar column (Rtx-Wax Restek, 30 m×0.25 mm i.d., 0.25 mm thickness) and a flame ionization detector, at the following temperatures: 80 °C (5 min), 80–200 °C (20 °C/min), 200 °C (5 min), 200–230 °C (5 °C/min), 230 °C (10 min), injector temperature at 250 °C, detector at 275 °C, with a 50:1 split ratio. Nitrogen and synthetic air (White Martins Gases Industriales Ltda., 99.99% purity) were the carrier gases, with a 1.5 mL/min flow rate. A diluted sample (0.4 mL) in dichloromethane (1:10; Merck) was injected. The sample components were identified by comparing their mass spectra with the mass spectra and retention indices of linolenate methyl ester (ω-3), linoleatemethyl ester (ω-6) and oleatemethyl ester (ω-9) standards (Sigma-Aldrich, Merck KGaA, St. Louis, MO, USA).

Oil extraction cost analysis

It is important to determine the relationship between the high extraction yield using propane and production costs. Based on the information generated by CONTAGRI software (16) the economic analysis generally depends on the total fixed and variable costs. In this way, the production costs included fixed and variable costs such as the raw material (seed), chemicals, reagents and gas (propane), equipment investment, and labour cost. Cost analysis of 16 working hours a day and 240 days a year of extraction unit operation took into consideration 1% for repair and maintenance, 10% for depreciation and 2% interest over a 10-year period.

Statistical analysis

The data from the experimental design were subjected to analysis of variance (ANOVA), and their average values were compared using the Tukey’s test at a 5% probability level for oil physicochemical analysis with Statistica v. 5.0 (17).

Results and Discussion

Extraction yield

Linseed oil extraction using organic solvent (n-hexane) gave a 36.1% yield in 14-hour extraction. Khattab and Zeitoun (14) obtained a 42.4% yield of linseed oil using the same extraction process; however, they did not mention the process extraction time. Table 1 shows the 22 experimental design matrix for linseed oil yield using subcritical extraction.

A 22 experimental design matrix with real and coded values and linseed oil yield results using subcritical propane and pressurized ethanol

Assay Independent variable Yield/%
Pressure/Pa Temperature/°C Subcriticalpropane Pressurizedethanol
1 –1 (8·106) –1 (20) 26.91 6.36
2 –1 (8·106) 1 (60) 27.57 6.10
3 1 (1.2·107) –1 (20) 27.05 6.93
4 1 (1.2·107) 1 (60) 28.31 7.23
5 0 (107) 0 (40) 28.39 7.99
6 0 (107) 0 (40) 28.12 7.89
7 0 (107) 0 (40) 28.29 8.05

High extraction yields were obtained in the central point (assays 5–7) and in assay 4 (Table 1). These results were similar to the ones obtained by Zanqui et al. (2), who verified a 28.8% yield of linseed oil using subcritical extractions with propane at 1.2·107 Pa and 60 °C. Pradhan et al. (3) and Khattab and Zeitoun (14) also reported similar extraction yields using supercritical CO2 respectively at 3·107 Pa and 50 °C for 4 h (35.3%) and 4·107 Pa and 50 °C for 2 h (36.9%). Such high yields might be due to high operational pressure and extraction time conditions, and solubility of the different compounds in the extraction solvent. In addition, the amount of solvent for complete extraction (12, 18, 19) was lower when using subcritical propane than when using supercritical CO2, making propane more attractive for industrial application.

Analysis of variance (ANOVA) served to see the effects of variables on the extraction yields of linseed oil using subcritical propane, observable in the Pareto chart (Fig. 1). Both temperature and pressure had significantly positive effects (p<0.1) on the extraction yield during 90-min extraction (Fig. 1).

Pareto chart for linseed oil extraction using subcritical propane at 90 min


The following equation shows the first order-coded model for extraction yield as a function of temperature and pressure within the studied ranges:Yield/%=27.8+0.48t+0.22p /2/where t is extraction temperature (°C) and p is extraction pressure (Pa).

The independent variables showed a positive effect (p<0.1), whereas the interactions were not significant. Analysis of variance (ANOVA) validated the model and the non-significant parameters were added to the lack of fit. The correlation coefficient was 0.9, and the F calculated value was 0.25 times higher than the F tabulated one (Table 2), allowing the contour curve construction (Fig. 2).

Analysis of variance for linseed oil extraction using subcritical propane

Source Sum of squares Degree of freedom Mean square F calculated
Recovery model 1.205 1 1.20 5.23
Residual 1.153 5 0.23
Lack-of-fit 1.115505 3
Pure error 0.037267 2
Total 2.357971 2

Surface response for linseed oil extraction using subcritical propane


Fig. 2 shows the increase of the extraction yield of linseed oil using subcritical propane with the increase of temperature and pressure.

Extraction using pressurized ethanol was performed under similar experimental conditions as the one using subcritical propane. The experimental design results (Table 1) showed that the highest yield (approx. 8%) was obtained at the central point (assays 5, 6 and 7). According to Table 1, the extraction using pressurized ethanol showed a lower yield than those using subcritical propane and organic solvent (n-hexane), which could be due to a short-chain alcohol that did not present any good solvency for triglycerides under the applied conditions. Besides that, this process requires, after extraction, an additional step for ethanol removal via evaporation, which is seen as a disadvantage, since it may cause loss or damage to certain oil components.

Jesus et al. (5) conducted a comparative study to evaluate palm oil extraction capacity with ethanol and propane under similar conditions (107 Pa and 20 °C) yielding 53.3% with ethanol and 72.8% with propane. The authors observed that the solubility obtained with propane was higher than with ethanol, allowing total oil miscibility, and improving extraction yield.

Statistically treated results of linseed oil extraction with pressurized ethanol showed that both pressure and its interaction with temperature had a significantly positive effect (p<0.1), observable in the Pareto chart (Fig. 3). The correlation coefficient was 0.92 and the F calculated was 1.44 times lower than the F tabulated value, hence, neither validating the model nor allowing the contour curve construction. Based on that, in order to increase the yield, the extraction time was prolonged to 3 h under the experimental design central point conditions (40 °C and 107 Pa). The improvement in the yield was 12.3%; however, even lower than in the extraction with subcritical propane (28.4%).

Pareto chart for linseed oil extraction using pressurized ethanol


Fatty acids

Table 3 shows the percentage of essential fatty acid esters obtained with different extraction methods from linseed oil, with methyl esters ranging from 49.8 to 55.0%.

Fatty acid ester values from brown linseed oil extracted using subcritical propane, pressurized ethanol, organic solvent, and mechanical pressing (commercial sample)

Experimental conditionp/Pa t/°C Yield/% Fatty acid ester
Linolenate (ω-3) Linoleate (ω-6) Oleate (ω-9)
Area/%
Extraction with subcritical propane
8·106 20 (26.9±1.0)a (53.7±0.4)b (13.1±0.7)a (19.2±0.6)b
8·106 60 (27.6±1.0)a (54.8 ±0.7)a (12.3±0.1)a (19.7±0.1)b
107 40 (28.1±1.0)a (54.6±0.5)a (12.0±0.3)a (21.0±0.2)a
1.2·107 20 (27.1±1.0)a (52.9±0.2)c (12.8±0.4)a (18.8±0.1)c
1.2·107 60 (28.3±1.0)a (54.5±0.4)a (11.7±0.7)a (20.3±0.5)a,b
Extraction with pressurized ethanol
8·106 20 (6.4±1.0)a (52.4±1.0)a (12.6±0.5)a (18.3±1.6)a
8·106 60 (6.1±1.0)a (51.1±0.3)a (13.0±0.1)a (22.2±2.3)a
107 40 (8.0±1.0)a (51.2±0.2)a (13.1±0.1)a (18.6±1.4)a
1.2·107 20 (7.0±1.0)a (53.4±2.0)a (13.6±0.6)a (18.7±1.2)a
1.2·107 60 (7.2±1.0)a (49.8±1.6)a (13.0±0.1)a (21.9±2.0)a
Extraction with organic solvent
105 68 36.1 55.0±0.2 12.4±0.2 19.2±1.0
Mechanical pressing
* 36.1 54.9±0.1 12.5±0.5 19.4±0.2

*Commercial sample. Same letters in columns in the same extraction method do not present any statistically significant difference according to the Tukey’s test (p<0.05)

Using propane as a solvent, Zanqui et al. (2) reported the yields higher than 48.7% ω-3 and 12.3% ω-6 from linseed oil. Results closer to those reported in this study were found by Ivanov et al. (20), who obtained 53.7% ω-3, 16.17% ω-6 and 19.4% ω-9 from linseed oil extracted using CO2, however, the used pressure ranged from 4.5 to 6.2·109 Pa, which may be explained by the extraction processing conditions and seed origin.

The statistical analysis of extraction using pressurized ethanol did not show any significant differences among acid esters, whereas the extraction with subcritical propane resulted in significant differences in linolenate (ω-3) and linoleate (ω-6) concentration. Extraction yields demonstrated that the concentrations of ω-3, -6 and -9 from linseed oil extracted using pressurized ethanol were similar to the ones found using subcritical propane (Table 3).

There were no statistical differences in the amounts of essential fatty acid esters obtained with the organic solvent and mechanical pressing, whose major component was linolenate (approx. 55%), corresponding to the ω-3 fatty acid, followed by oleate (19%) (matching the ω-9 fatty acid). Such results corroborated those found in the literature. Pradhan et al. (3) obtained 53.8% ω-3 and 15.6% ω-6 from linseed oil extracted using mechanical pressing and 50% ω-3 and 14.4% ω-6 in the oil extracted using organic solvent. Linseed oil extracted with n-hexane contained 56.28 and 15.6% ω-3 and ω-6, respectively (14).

The area sum (%) of fatty acid esters (linolenate (ω-3), linoleate (ω-6) and oleate (ω-9)) was around 86% (Table 3). The remaining 14% may be related to the amounts of both palmitic and stearic acids. Studies in the literature of fatty acid composition from linseed oil showed 45.1–73.4% linolenic, 11.9–20% linoleic, 17.1–24.3% oleic, 4.6–6.9% palmitic and 0.25–4.6% stearic acids (21-23).

In this work, the ω-6/ω-3 family polyunsaturated fatty acid ratio was around 0.24. An ω-6 to ω-3 essential fatty acid ratio of approx. 1 is considered nutritionally adequate (24). A lower ω-6/ω-3 fatty acid ratio is more desirable in reducing the risk of chronic diseases since polyunsaturated fatty acids are considered essential to the human body with nutritional advantages as long chain fatty acid (linoleic and linolenic acids) precursors. They can improve the brain, heart and immune system performance in humans, as well as have suppressive effects on the onset of cardiovascular, inflammatory and cancer diseases (24, 25).

The main advantage of extraction with subcritical propane are milder operating conditions than with supercritical CO2. The use of propane reduced drastically operation pressure, and enabled its industrial application based on a cost analysis for implementation feasibility.

Physicochemical characterization

Physicochemical analyses are crucial for oil quality evaluation. Table 4 shows brown linseed oil acidity, refraction indices and density values after different extraction methods. The samples with high yield (central point, assays 5, 6 and 7) were used for extractions with subcritical propane and pressurized ethanol.

Brown linseed oil physicochemical analysis results

Extraction Analysis
Density/(g/cm3) Acidity/% Refraction index (40 °C)
Mechanical press (0.90±0.01)a (1.40±0.04)c (1.40±0.05)a
n-Hexane (0.90±0.01)a (1.50±0.01)b (1.40±0.04)a
Propane (0.90±0.01)a (0.90±0.01)d (1.40±0.01)a
Ethanol (0.90±0.01)a (1.70±0.02)a (1.40±0.01)a

*Same letters in columns do not represent any statistically significant difference according to the Tukey’s test (p<0.05)

As observable in Table 4, the density and refractive indices were not statistically significant (p<0.05) for all linseed oil samples using the different extraction methods, as confirmed by Tukey’s test, with 95% confidence level. According to the Adolfo Lutz Institute, Brazil (15), the refraction index is a good oil quality indicator and is affected by oxidation, heat treatment and free fatty acid content from oil. Gunstone et al. (26) and Firestone (27) recommend values from 0.924 to 0.930 g/cm3.

Linseed oil acidity was statistically different among all extraction methods studied. The highest (1.76%) and lowest (0.95%) values were obtained using pressurized ethanol and subcritical propane, respectively. The highest acidity values were found after extractions with organic solvent (1.46%) and pressurized ethanol (1.75%). Such results were obtained since heating was used in both methods. Extractions using n-hexane (68 °C) and pressurized ethanol have a solvent evaporation step after the extraction. Heating can cause a triglyceride chain break during extraction, hence increasing acidity through fatty acid release (28). Thus, the physicochemical parameters indicate that oil extraction using subcritical propane was preferable due to fatty acid quality with low acidity.

Cost analysis of the process

The oil yield obtained with extraction using subcritical propane was around 28.3%. Table 5 (29) shows fixed and variable costs, as well as oil total cost per litre. Fixed oil unit production cost was around US$ 56 540.00 and plant age was approx. 10 years. The total oil cost per litre is high when produced in a laboratory scale unit, as it yields small quantities of linseed oil (0.48 L/day).

Production costs of 1 L linseed oil extracted with propane

Item Quantity Amount (US$)
Fixed cost
Extractor unit 1 6000.00
Oven dryer 1 200.00
Mixer 1 40.00
Freezer 1 300.00
Building 50000.00
Repair and maintenance (1%) 5654.00
Depreciation (10%) 565.40
Interest (2%) 113.08
Total 62872.48
Cost/L (0.48 L/day) 4.62
Variable cost
Seed cost (monthly cost) 0.17 kg/day 1.63*
Electricity (monthly cost) 50.00
Chemicals, reagents and gas (monthly cost) 100.00
Labour (skilled and operator helpers) (monthly cost) 2 1000.00
Total 1151.63
Cost/L (0.48L/day) 119.96
Total cost per litre 124.58

*Seed cost was based on Flax Council of Canada International Market (29)

Galvão et al. (30) reported a US$ 13.21/kg specific cost when evaluating the supercritical technology (CO2 solvent) costs for flaxseed oil extraction, whereas linseed oil obtained by cold mechanical pressing is marketed at US$ 54.00/kg in Brazil (31). These costs when compared to those for propane extraction make it a non-market-competitive technology. However, industrial-scale propane extraction (increased capacity) could reduce subcritical extraction costs, making it an attractive method.

CONCLUSIONS

Physicochemical analyses of density and refractive index of oil samples obtained using the different extraction methods were similar. All extraction methods gave similar fatty acid ester profile. The subcritical propane was an efficient method for brown linseed oil extraction achieving good yield, maintaining the quality and quantity of fatty acids at mild temperature and pressure conditions, and completly removing the solvent from the final product. Since extraction using subcritical propane was economically feasible on a laboratory scale, an industrial scale process is recommended for capacity increase.

ACKNOWLEDGEMENTS

ACKNOWLEDGEMENTS

The authors greatly appreciate the help of their colleagues at the Department of Food Engineering, URI Erechim, RS, and CAPES, Erechim, Brazil.

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