α-Amylase Production by Bacillus amyloliquefaciens Using Agro Wastes as Feed Stock

Dhanya Gangadharan, K. Madhavan Nampoothiri* and Ashok Pandey

Biotechnology Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, 695 019 Trivandrum, India

Article history:

Received September 13, 2010
Accepted February 15, 2011

Key words:

α-amylase, Bacillus amyloliquefaciens, fermentor, bioreactor, aeration, submerged fermentation


The productivity of enzyme fermentations depends critically on maintaining a high oxygen transfer rate to satisfy the optimal oxygen demand of the microorganism for product formation. Among the several factors that affect oxygen transfer rates in a fermentor are the air flow rate and agitation. The production of α-amylase by Bacillus amyloliquefaciens was performed in 600-mL and 5-litre fermentor with a working volume of 300 mL and 3 L, respectively. The experiments indicated a requirement of high rates of aeration to enhance the enzyme yield. The biomass yield and productivity of the enzyme were found to have a linear relationship with the air flow rate, and the highest productivity was observed at 1.0 vvm. A maximum productivity of 41.4 U/(mL·h) was obtained after 14 h of fermentation in 600-mL fermentor system and a comparable productivity of 40 U/(mL·h) was obtained after 12 h in the 5-litre fermentor.

*Corresponding author: 
                                               ++91 471 251 5366
++91 471 249 1712

Search FTB

Follow us

 facebook 1 twitter bird_icon LI In Bug


QR Code


We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information