Cysteine Protease (Capparin) from Capsules of Caper (Capparis spinosa)

Yasar Demir1, Azize Alayli Güngör2, Elif Duygu Duran3 and Nazan Demir3*

1Department of Chemistry, College of Education, Atatürk University, TR-25240 Erzurum, Turkey

2Refik Saydam Institute of Hygiene, Headmaster of Erzurum Region, TR-25100 Erzurum, Turkey
3Department of Chemistry, College of Arts and Sciences, Atatürk University, TR-25240 Erzurum, Turkey

Article history:

Received December 20, 2006
Accepted March 19, 2008

Key words:

protease, caper (Capparis spinosa), food production


Proteases are enzymes that perform very important functions in organisms and are used for a variety of objectives in vitro. In recent years, proteases have been used for clinical, pharmaceutical (alimentary digestion, anti-inflammatory, etc.) and industrial applications (cheese production, meat tenderizing, leather tanning). In this research, a protease has been purified from capsules of caper (Capparis spinosa) and characterized. Caper plants have been used for food and medicine since ancient times. The plant grows abundantly in certain regions of Turkey. Ammonium sulphate fractionation and a CM Sephadex column were used for purification of the enzyme. The purification enzyme has an optimum pH=5.0 and its optimum temperature was 60 °C. The vmax and Km values determined by Lineweaver-Burk graphics were 1.38 μg/(L·min) and 0.88 μg/L, respectively. The purification degree and the molecular mass of the enzyme (46 kDa) were determined by SDS-PAGE and gel filtration chromatography. It was investigated whether the purified and characterized protease could cause milk to congeal or digest chicken and cow meat. The results show that protease can be used for industrial production.


*Corresponding author: 
                                               ++90 442 2314 439
                                               ++90 442 2360 948

Search FTB

Follow us

 facebook 1 twitter bird_icon LI In Bug


QR Code


We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information