Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B

Nándor Nemestóthy*, László Gubicza, Erika Fehér and Katalin Bélafi-Bakó

Research Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, Hungary

Article history:

Received July 4, 2006
Accepted February 6, 2007

Key words:

fusel oil, esterification, lipase, ping-pong bi-bi mechanism


Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping-pong bi-bi mechanism (inhibition phenomena taken into account) was applied as a complex kinetic model. The parameters of the model were determined by numerical methods. It was found that four-parameter model fitted well with the experimental results and described properly the enzymatic reaction.


*Corresponding author: 
                                               ++36 88 624 044
                                               ++36 88 624 038

Search FTB

Follow us

 facebook 1 twitter bird_icon LI In Bug


Environmental Policy

sdg publishers compact 4 300x300

QR Code


We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information