Preparing Apigenin from Leaves of Adinandra nitida

Benguo Liu*, Zhengxiang Ning, Jianhua Gao and Keyong Xu

College of Light Industry and Food Science, South China University of Technology, Guangzhou, Guangdong, CN-510640, PR China

Article history:

Received February 17, 2006
Accepted July 6, 2006

Key words:

Adinandra nitida, flavonoid, apigenin, electrospray ionization mass spectrometry


Leaves of Adinandra nitida were used as raw material, and a new industrially significant method of preparing apigenin was established by hydrolyzing a water extract and recrystallizing it with ethanol in order to obtain a new source for the production of this flavone. A yield of about 2.5 % (dry mass) was obtained with the purity of 93.05 %, determined by high performance liquid chromatography (HPLC). Moreover, the main flavonoids in leaves of Adinandra nitida and the product after acid hydrolysis were identified as camellianin A and apigenin, respectively, by ultraviolet-visible spectrometry (UV/VIS) and electrospray ionization mass spectrometry (ESI-MS).

*Corresponding author: 
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