Antimutagenic Properties of Basil (Ocimum basilicum L.) in Salmonella typhimurium TA100

Olivera Stajković1, Tanja Berić-Bjedov2*, Dragana Mitić-Ćulafić2, Slaviša Stanković2, Branka Vuković-Gačić2, Draga Simić2 and Jelena Knežević-Vukčević2

Laboratory for Microbiology, Institute of Soil Science, Teodora Drajzera 7, 11000 Belgrade, Serbia

2Laboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia

Article history:

Received November 4, 2005
Accepted June 7, 2006

Key words:

4-nitroquinoline-N-oxide (4NQO), 2-nitropropane (2-NP), benzo(a)pyrene (B(a)P), UVC irradiation, Ocimum basilicum, antimutagens, Salmonella typhimurium TA100


The use of dietary antimutagens and anticarcinogens has been seen as a promising approach to the protection of human health. Basil (Ocimum basilicum L.) is a well-known medicinal and aromatic plant, with a range of newly discovered biological activities possibly important for chemoprevention. In the preliminary experiments, toxic and mutagenic potential of essential oil (EO) from basil and pure substances: linalool, β-myrcene and 1,8-cineole were tested using Salmonella typhimurium TA98, TA100 and TA102, with and without S9 mix (microsomal fraction of rat liver). No mutagenic effect of basil derivatives was detected in any tested strain. Antimutagenic effects of essential oil from basil and its pure constituents were further evaluated in the Ames test using S. typhimurium TA100. UVC irradiation and three chemical mutagens, 4-nitroquinoline-N-oxide (4NQO), 2-nitropropane (2-NP) and benzo(a)pyrene (B(a)P) were used to induce mutagenesis. All tested basil derivatives significantly reduced UV-induced mutations. The maximum inhibition was in the range of 64–77 %. Inhibitory potential against direct acting model mutagen/carcinogen 4NQO was similar to UV (52–67 %). In the presence of S9, EO and 1,8-cineole showed moderate inhibition of 2-NP induced mutagenesis, while the remaining two substances had no effect. Linalool exhibited high co-mutagenic effect with B(a)P, 1,8-cineole showed moderate inhibitory effect against B(a)P-induced mutations, while EO and β-myrcene were ineffective.


*Corresponding author: 
                                               ++381 11 637 364
                                               ++381 11 637 364

Search FTB

Follow us

 facebook 1 twitter bird_icon LI In Bug


QR Code


We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information