Biotechnological Potential of Yarrowia lipolytica Grown under Thiamine Limitation

Svetlana V. Kamzolova1*, Maria N. Chiglintseva1,2, Alsu I. Yusupova1, Natalia G. Vinokurova1, Valentina Ya. Lysanskaya1 and Igor G. Morgunov1

1G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia

2Pushchino Branch of Moscow State University, Microdistrict 'V' 25, Pushchino, RU-142290 Moscow Region, Russia

Article history:

Received June 15, 2011
Accepted November 17, 2011

Key words:

microbial production, succinic acid, α-ketoglutaric acid, Yarrowia lipolytica, γ-aminobutyric acid, diethyl succinate


During the cultivation of a thiamine-auxotrophic yeast strain Yarrowia lipolytica VKM Y-2412 on ethanol, the growth limitation by thiamine leads to the production of α-ketoglutaric acid. The α-ketoglutaric acid synthesis has been studied in dependence on pH, oxygen supply and ethanol, zinc and iron concentrations. Under optimal conditions, Y. lipolytica produced 88.7 g/L of α-ketoglutaric acid. The culture broth containing α-ketoglutaric acid was subjected to chemical treatment with hydrogen peroxide, which led to the formation of succinic acid in significant quantities (71.7 g/L). Further direct esterification of succinic acid with excess absolute ethanol yielded diethyl succinate. Biomass of Y. lipolytica, a superproducer of α-ketoglutaric acid, was characterized by a high content of protein and essential amino acids, free amino acids, and unusually large amount of γ-aminobutyric acid. The unique amino acid composition of the producer makes it possible to use this biomass as a component of parenteral nutrition mixtures and as a basis for neuroleptics.

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