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Streptomyces rimosus GDS(L) Lipase: Production, Heterologous Overexpression and Structure-Stability Relationship

Dušica Vujaklija1*, Marija Abramić1, Ivana Leščić1, Tereza Maršić2 and Jasenka Pigac1


1
Ruđer Bošković Institute, Department of Molecular Genetics, Department of Organic Chemistry and Biochemistry, Department of Physical Chemistry, Bijenička cesta 54, HR-10002 Zagreb, Croatia

2PLIVA Research Institute, Therapeutic Area 2, Prilaz Baruna Filipovića 25, HR-10000 Zagreb, Croatia

Article history:

Received: December 4, 2002
Accepted: January 24, 2003

Key words:

lipase, Streptomyces, plate assay, thermostability

Summary:

Streptomyces rimosus lipase gene has been overexpressed in a heterologous host, S. lividans TK23. The maximal lipase activity was determined in the culture filtrates of the late stationary phase. Time course of lipase production was monitored by a modified plate assay. S. rimosus lipase gene has been located on the AseI B fragment approximately 2 Mb far from the left end of the S. rimosus linear chromosome. Out of eight examined streptomycetes, the presence of this rare type of bacterial lipase gene was detected in two belonging to the S. rimosus taxonomic cluster, and in one non-related species. Comparison of protein sequences of the Streptomyces lipolytic enzymes was performed. The result indicated the best structural stability of the putative S. coelicolor lipase-2. 



*Corresponding author:           vujaklij@rudjer.irb.hr
                                               ++385 (0)1 4561-111/1459
                                               ++385 (0)1 4561-177

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