Screening of Ipomoea tuba Leaf Extract for Identification of Bioactive Compounds and Evaluation of Its in vitro Antiproliferative Activity Against MCF-7 and HeLa Cells
Thirupati Chinna Venkateswarulu1, Gaddam Eswaraiah1, Srirama Krupanidhi1, Karlapudi Abraham Peele1, Indira Mikkili1, Alugunulla Venkata Narayana1, Bharath Kumar Ravuru2, John Babu Dulla1 and Ranga Rao Ambati1*
1Department of Bio-Technology, Vignan’s Foundation for Science, Technology & Research (Deemed to be University), Vadlamudi-522213, Guntur, Andhra Pradesh, India
2School of Life Sciences, Rayalaseema University, Kurnool-518002, Andhra Pradesh, India
Received: 2 May 2019
Accepted: 17 March 2020
mangrove species, Ipomoea tuba, bioactive compounds, antiproliferative activity
Mangroves contain a wide range of bioactive compounds with pharmacological activities.In the present study, we analysed the separation and detection of phytoconstituentswith the methanol extract of Ipomoea tuba leaf using gas chromatography-massspectrometry (GC-MS) and tested its in vitro cytotoxicity effect against MCF-7 and HeLacells. Phytochemical compounds such as docosanoic, octadecatrienoic and cis-9-octadecanoicacids, triterpenoid γ-sitosterol, and terpene alcohol in methanol extract of I. tubaleaf were identified. Furthermore, in vitro antiproliferative activity of the extract of I. tubaleaf was evaluated using MCF-7 and HeLa cells. The results indicated a reduction of cell viabilityof 37.43 and 41.89 % of MCF-7 and HeLa cells respectively. The methanol extract ofI. tuba leaf proved to be effective in protecting the cells against oxidative stress. This is thefirst report on the in vitro cytotoxicity effect of I. tuba leaf extract on MCF-7 and HeLa cells.