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Modelling the Inactivation and Possible Regrowth of Salmonella enterica Treated with Chlorophyllin-Chitosan Complex and Visible Light

María Isabel Rodríguez-López1orcid tiny, Vicente M. Gómez-López2orcid tiny, Viktorija Lukseviciute3orcid tiny and Zivile Luksiene3*orcid tiny

1Departamento de Ciencia y Tecnología de Alimentos, Universidad Católica de Murcia (UCAM), Campus de los Jerónimos 135, 30107 Guadalupe, Murcia, Spain
2Cátedra Alimentos para la Salud, Universidad Católica de Murcia (UCAM), Campus de los Jerónimos 135, 30107 Guadalupe, Murcia, Spain
3Institute of Computer Science, Vilnius University, Didlaukio g. 47, 08303 Vilnius, Lithuania
4Institute of Photonics and Nanotechnology, Vilnius University, Saulėtekio 10, 10223 Vilnius, Lithuania

Article history:
Received: 20 May 2019
Accepted: 17 February 2020
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Key words:
photosensitization, microbial modelling, treatment with chlorophyllin and chitosan complex, Salmonella enterica, microbial inactivation

The study focuses on predictive modelling of inactivation of Salmonella enterica after treatment with chlorophyllin-chitosan complex and visible light. Salmonella cells were incubated with chlorophyllin-chitosan complex (0.001 % chlorophyllin and 0.1 % chitosan) for different times (5-60 min) and then illuminated with visible light (λ=405 nm, He=38 J/cm2). Inactivation curves and post-treatment regrowth curves were built based on microbiological viability tests and data were fitted to ten inactivation and two regrowth models. The photoactivated complex reduced Salmonella population, which were unable to regrow. Weibull and Baranyi models were the best to describe the inactivation and regrowth kinetics respectively. In conclusion, data from the kinetic analysis and predictive modelling confirmed that photoactivated chlorophyllin-chitosan complex is a promising non-thermal approach for inactivation of Gram-negative pathogens, since no bacterial regrowth after treatment has been predicted.

*Corresponding author: tel3  +3702195030 
                                           fax2  +3702151585 

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