getpdf   NLM PubMed Logo   https://doi.org/10.17113/ftb.58.01.20.6374  

Modelling the Inactivation and Possible Regrowth of Salmonella enterica Treated with Chlorophyllin-Chitosan Complex and Visible Light

María Isabel Rodríguez-López1orcid tiny, Vicente M. Gómez-López2orcid tiny, Viktorija Lukseviciute3orcid tiny and Zivile Luksiene3*orcid tiny

1Departamento de Ciencia y Tecnología de Alimentos, Universidad Católica de Murcia (UCAM), Campus de los Jerónimos 135, 30107 Guadalupe, Murcia, Spain
2Cátedra Alimentos para la Salud, Universidad Católica de Murcia (UCAM), Campus de los Jerónimos 135, 30107 Guadalupe, Murcia, Spain
3Institute of Computer Science, Vilnius University, Didlaukio g. 47, 08303 Vilnius, Lithuania
4Institute of Photonics and Nanotechnology, Vilnius University, Saulėtekio 10, 10223 Vilnius, Lithuania

Article history:
Received: 20 May 2019
Accepted: 17 February 2020
cc by

Key words:
photosensitization, microbial modelling, treatment with chlorophyllin and chitosan complex, Salmonella enterica, microbial inactivation

Summary:
The study focuses on predictive modelling of inactivation of Salmonella enterica after treatment with chlorophyllin-chitosan complex and visible light. Salmonella cells were incubated with chlorophyllin-chitosan complex (0.001 % chlorophyllin and 0.1 % chitosan) for different times (5-60 min) and then illuminated with visible light (λ=405 nm, He=38 J/cm2). Inactivation curves and post-treatment regrowth curves were built based on microbiological viability tests and data were fitted to ten inactivation and two regrowth models. The photoactivated complex reduced Salmonella population, which were unable to regrow. Weibull and Baranyi models were the best to describe the inactivation and regrowth kinetics respectively. In conclusion, data from the kinetic analysis and predictive modelling confirmed that photoactivated chlorophyllin-chitosan complex is a promising non-thermal approach for inactivation of Gram-negative pathogens, since no bacterial regrowth after treatment has been predicted.

*Corresponding author: tel3  +3702195030 
                                           fax2  +3702151585 
                                            email3  zivile.luksiene@tmi.vu.lt 

Search FTB

Follow us

 facebook 1 twitter bird_icon LI In Bug

 

QR Code

qrcode

We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information