getpdf NLM PubMed Logo https://doi.org/10.17113/ftb.59.03.21.6934  Supplement 

Optimization of β-Fructofuranosidase Production from Agrowaste by Aspergillus carbonarius and Its Application in the Production of Inverted Sugar

Ryhára Dias Batista1orcid tiny, Fernanda Guimarães Melo1, Claudia Cristina Auler do Amaral Santos1orcid tiny, Fabrício Coutinho de Paula-Elias1orcid tiny, Rafael Firmani Perna2orcid tiny, Michelle Cunha Abreu Xavier3orcid tiny, Sergio Andres Villalba Morales2orcid tiny and Alex Fernando de Almeida1*orcid tiny

1Graduate Program in Food Science and Technology, Federal University of Tocantins, 109 Norte Av. NS-15, ALCNO-14, Plano Diretor Norte, CEP: 77001-090, Palmas, Tocantins, Brazil

2Federal University of Alfenas (UNIFAL-MG), Institute of Science and Technology, José Aurélio Vilela Road 11999, Km 533, Zip Code 37715-400, Poços de Caldas, MG, Brazil

3Federal University of Tocantins (UFT), Department of Bioprocess Engineering and Biotechnology, Badejos Street 69-72, Jardim Cervilha, Zip Code 77404-970, Gurupi, TO, Brazil

Article history:

Received: 8 August 2020

Accepted: 14 July 2021

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Key words:

invertase, Aspergillus carbonarius PC-4, culture optimization, simplex lattice design, sucrose hydrolysis 

Summary:

Research backgroundMicrobial β-fructofuranosidases are widely employed in food industry to produce inverted sugar or fructooligosaccharides. In this study, a newly isolated Aspergillus carbonarius PC-4 strain was used to optimize the β-fructofuranosidase production in a cost-effective process and the sucrose hydrolysis was evaluated to produce inverted sugars.

Experimental approachOptimization of nutritional components of culture medium was carried out using simplex lattice mixture design for 72 and 120 h at 28 °C. One-factor-at-a-time methodology was used to optimize the physicochemical parameters. Crude enzyme was used for sucrose hydrolysis at different concentrations. 

Results and conclusionsThe optimized conditions of enzyme production were achieved from cultivations containing pineapple crown waste (1.3 %, m/V) and yeast extract (0.3 % m/V) after 72 h with an enzyme activity of 9.4 U/mL, obtaining R²=91.85 %, R²adjusted=85.06 %, highest F-value (13.52) and low p-value (0.003). One-factor-at-a-time used for optimizing the physicochemical conditions showed optimum temperature (20 °C), pH (5.5), agitation (180 rpm) and time course (72 h) with a 3-fold increase of enzyme production. The invertase-induced sucrose hydrolysis showed the maximum yield (3.45 mmol of reducing sugars) using 10 % of initial sucrose concentration. Higher sucrose concentrations caused the inhibition of invertase activity, possibly due to the saturation of substrate or formation of sucrose aggregates, making it difficult for the enzyme to access sucrose molecules within the created clusters. Therefore, a cost-effective method was developed for the invertase production using agroindustrial waste and the produced enzyme can be used efficiently for inverted sugar production at high sucrose concentration.

Novelty and scientific contributionThis study presents an efficient utilization of pineapple crown waste to produce invertase by a newly isolated Aspergillus carbonarius PC-4 strain. This enzyme exhibited a good potential for inverted sugar production at high initial sucrose concentration, which is interesting for industrial applications.

*Corresponding author: +556333111604
  alexfernando@uft.edu.br

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