doi: 10.17113/ftb.55.01.17.4812 

An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

Magdalena Rakicka*, Aleksandra M. Mirończuk, Ludwika Tomaszewska-Hetman, Anita Rywińska and Waldemar Rymowicz
 
 

Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Chełmońskiego St. 37, PL-51-630 Wroclaw, Poland

Article history:
Received  June 3, 2016
Accepted  November 7, 2016

Key words:
erythritol, glycerol, chemostat, Yarrowia lipolytica


 

Summary:
This study demonstrates the potential applicability of the UV mutant Yarrowia lipolytica MK1 for the valorisation of glycerol and erythritol production in a chemostat culture. The aim of this research is to investigate the optimal C:N ratio in the feeding medium in order to enhance erythritol production. The highest erythritol concentration, at 113.1 g/L with a volumetric erythritol production rate of 1.1 g/(L·h) and a yield of 0.57 g/g, was obtained in the feeding medium with a C:N ratio of 80:1. Moreover, no residual glycerol was observed in the culture broth during cultivation. The chemical composition of the biomass was analysed. The contents of lysine and threonine in the biomass protein amino acid profile were higher than those required by the FAO/WHO for fodder yeast.



 

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