Production of α-Amylase from Penicillium chrysogenum under Solid-State Fermentation by Using Some Agricultural By-Products

Bilal Balkan and Figen Ertan*

Trakya University, Science and Art Faculty, Department of Biology, TR-22 080 Edirne, Turkey

Article history:
Received May 23, 2006
Accepted November 6, 2006

Key words:
α-amylase, Penicillium chrysogenum, solid-state fermentation

Summary:
Solid-state fermentation (SSF) was carried out using corncob leaf (CL), rye straw (RS),wheat straw (WS) and wheat bran (WB) as substrates for α-amylase production by a fungalculture of Penicillium chrysogenum. The effects of moisture level, particle size and inoculum concentration on enzyme synthesis from P. chrysogenum were investigated. Optimal moisture levels of substrates were 75, 65, 65 and 55 % for CL, WS, WB and RS substrates, respectively. Optimal particle size and inoculum concentration for the production of α-amylase were: >1 mm, 20 %; >1 mm, 20 %; 1 mm, 20 % and >1 mm, 30 % for CL, WS, WB and RS, respectively. WB showed the highest enzyme production with 160 U/mL under optimum conditions. The other enzyme activities were 28, 49 and 45 U/mL using CL, RS and WS, respectively.

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