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A Lysozyme with Antifungal Activity from Pithecellobium dulce Seeds

Narumon Sawasdipuksa1, Zhentian Lei2, Lloyd W. Sumner2, Ploypat Niyomploy3 and Polkit Sangvanich1*


1Research Centre for Bioorganic Chemistry, Department of Chemistry, Faculty of Science,
Chulalongkorn University, Bangkok 10330, Thailand
2Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore 73401, Oklahoma, USA
3Biotechnology Program, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand

Article history:

Received January 15, 2010
Accepted June 16, 2010

Keywords:

Pithecellobium dulce, seed protein, lysozyme, antifungal activity

Summary:

A protein of an apparent molecular mass of 14.4 kDa with antifungal activity was isolated from the seeds of Pithecellobium dulce using extraction with 100 mM Tris-HCl buffer (pH=8.0), precipitation with 80 % ammonium sulfate, and bioassay purification via Resource Q anion exchange chromatography and Superdex 200 gel filtration chromatography. The purified protein was putatively identified by tandem mass spectrometry with Mascot database searching, with the partial amino acid sequences showing a high degree of similarity to chicken egg white lysozyme. This putative plant lysozyme expressed antifungal activity with a rather high thermal stability of up to 80 °C for 15 min (at pH=8.0). It exerted an antifungal action towards Macrophomina phaseolina but displayed no antifungal activity against two other isolates, Phymatotrichopsis omnivora and Fusarium avenaceum.

 


*Corresponding author:           dunja@zg.biol.pmf.hr
                                               ++385 1 460 6263
                                               ++385 1 460 6286

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