Solid-State Fermentation of Carrot Pomace for the Production of Inulinase by Penicillium oxalicum BGPUP-4

Ram Sarup Singh1*small orcid_display_4pp, Kanika Chauhan1small orcid_display_4pp, Jagroop Singh1small orcid_display_4pp, Ashok Pandey2
small orcid_display_4pp and Christian Larroche3small orcid_display_4pp



1Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, 147002 Patiala, Punjab, India
2CSIR-Indian Institute of Toxicology Research, 31 Marg, 226001 Lucknow, India
3University Clermont Auvergne, Institute Pascal, UMR, CNRS 6602, and LabEx IMobS3, 4 avenue Blaise Pascal, TSA 60026, CS 60026, FR-63178, Aubiere cedex, France

Article history:
Received: July 5, 2017
Accepted: November 2, 2017

Key words:
inulinase, carrot pomace, Penicillium oxalicum, solid-state fermentation, response surface methodology

Inulinases are an important class of industrial enzymes which are used for the production of high-fructose syrup and fructooligosaccharides. Inulin, a polyfructan, is generally employed for the production of inulinase, which is a very expensive substrate. A number of agroindustrial residues have been used for cost-effective production of inulinases. In the present study, carrot pomace was selected as a substrate for the production of inulinase by Penicillium oxalicum BGPUP-4 in solid-state fermentation. Carrot pomace is one of the good substrates for bioprocesses, because it is rich in soluble and insoluble carbohydrates. A central composite rotatable design (CCRD) used in response surface methodology was employed for the optimal production of inulinase from carrot pomace. Using CCRD, 15 runs were practiced to optimize the range of three independent variables: moisture content (70-90 %), incubation time (4-6 days) and pH (5.0-7.0) for inulinase production. Carrot pomace supplemented with 0.5 % inulin as an inducer, 0.2 % NH4H2PO4, 0.2 % NaNO3, 0.2 % KH2PO4, 0.05 % MgSO4·7H2O and 0.001 % FeSO4·7H2O was used for the production of inulinase in solid-state fermentation at 30 °C. Inulinase production (322.10 IU per g of dry substrate) was obtained under the optimized conditions, i.e. moisture content of 90 %, incubation time 4 days and pH=7.0. The corresponding inulinase/invertase (I/S) ratio (3.38) was also high, which indicates the inulolytic nature of the enzyme. Multiple correlation coefficients R for inulinase production and I/S ratio were 0.9995 and 0.9947, respectively. The R value very close to one indicates an excellent correlation between experimental and predicted results.

*Corresponding author:  tel3  
                                                     fax2  +911752283073

Paper was presented at the 7th International Forum on Industrial Bioprocessing - IFIBiop 2017, May 21-24, 2017, Wuxi, PR China