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Optimization of Process Parameters for the Production of γ-Linolenic Acid by Cunninghamella elegans CFR C07 in Submerged Fermentation


Parvathy Sree Varma1#small orcid_display_4pp, Salini Chandrasekharan1#small orcid_display_4pp
, Govindarajulu Venkateswaran2small orcid_display_4pp, Santhosh Rajendran2small orcid_display_4pp, Kiran Kumar Mallapureddy1small orcid_display_4pp, Ashok Pandey1,3small orcid_display_4pp and Binod Parameswaran1*small orcid_display_4pp




1Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., 695019 Thiruvananthapuram, Kerala, India
2CSIR-Central Food Technological Research Institute, Kajjihundi, 570020 Mysore, Karnataka, India
3CSIR-Indian Institute of Toxicology Research, 31 MG Marg, 226001 Lucknow, India




Article history:
Received: August 4, 2017
Accepted: January 17, 2018




Key words:
γ-linolenic acid, submerged fermentation, Cunninghamella elegans, fatty acid production




Summary:
The production of γ-linolenic acid (GLA) by the fungus Cunninghamella elegans CFR C07 in submerged fermentation was studied. Culture parameters such as carbon source and incubation time were optimized. Four different extraction methods using solvents with acid washed sand, glass beads, lyophilization and Soxhlet extraction were evaluated for improved extraction of lipids from the fungal biomass after fermentation. The GLA production was initially optimized in 250-mL flask and the process was demonstrated in a 3-litre fermentor. The maximum GLA production was 882 mg/L in shake flask culture and 733 mg/L in the fermentor. The study shows that Cunninghamella elegans CFR C07 is a potent organism for the production of GLA under submerged conditions.




*Corresponding author:  tel3  +914712515361
                                                     fax2  +914712491712
                                                      email3  binodkannur@niist.res.in



#
Both authors contributed equally




Paper was presented at the 7th International Forum on Industrial Bioprocessing - IFIBiop 2017, May 21-24, 2017, Wuxi, PR China