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Enzymatic Conversion of Sugar Beet Pulp: A Comparison of Simultaneous Saccharification and Fermentation and Separate Hydrolysis and Fermentation for Lactic Acid Production


Joanna Berlowska1*orcid tiny, Weronika Cieciura-Włoch1orcid tiny, Halina Kalinowska2orcid tiny, Dorota Kregiel1orcid tiny, Sebastian Borowski1orcid tiny, Ewelina Pawlikowska1orcid tiny, Michał Binczarski3orcid tiny and Izabela Witonska3orcid tiny




1Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, PL-90-924 Lodz, Poland
2Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego 4/10, PL-90-924 Lodz, Poland
3Institute of General and Ecological Chemistry, Lodz University of Technology, Zeromskiego 116, PL-90-924 Lodz, Poland



Article history:
Received: 15 June 2017
Accepted: 30 January 2018



Key words:
sugar beet pulp, enzymatic hydrolysis, lactic acid



Summary:
This study compares the efficiency of lactic acid production by separate hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF) of sugar beet pulp, a byproduct of industrial sugar production. In experiments, sugar beet pulp was hydrolyzed using five commercial enzymes. A series of shake flask fermentations were conducted using five selected strains of lactic acid bacteria (LAB). The differences in the activities of the enzymes for degrading the principal sugar beet pulp components were reflected in the different yields of total reducing sugars. The highest yields after hydrolysis and the lowest quantities of insoluble residues were obtained using a mixture (1:1) of Viscozyme® and Ultraflo® Max. In the SHF process, only a portion of the soluble sugars released by the enzymes from the sugar beet pulp was assimilated by the LAB strains. In SSF, low enzyme loads led to reduction in the efficiency of sugar accumulation. The risk of carbon catabolic repression was reduced. Our results suggest that SSF has advantages over SHF, including lower processing costs and higher productivity. Lactic acid yield in SSF mode (approx. 30 g/L) was 80–90 % higher than that in SHF.



*Corresponding author:  tel3  +48426313480
                                            email3  joanna.berlowska@p.lodz.pl

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