Expression of Aspergillus oryzae Tannase in Pichia pastoris and Its Application in the Synthesis of Propyl Gallate in Organic Solvent

Xiao-Wei Yu^1,2 and Yong-Quan Li¹*

¹Zhejiang University College of Life Science, CN-310058 Hangzhou, PR China
²Jiangnan University College of Biotechnology, CN-214122 Wuxi, PR China

Article history:
Received November 27, 2006
Accepted October 2, 2007

Key words:
tannase, Pichia pastoris, recombinant protein production, propyl gallate, biosynthesis

Summary:
Gallic acid esters could be synthesized biologically by tannase in organic media, among which is propyl gallate, an antioxidant widely used as a food additive. Efficient intracellular expression of Aspergillus oryzae tannase was achieved in Pichia pastoris under the control of the AOX1 promoter, and the productivity of recombinant tannase was 960 U/L or 64 U/g dry mass. The recombinant P. pastoris was used to synthesize propyl gallate in organic solvent and the yield of propyl gallate was 53 %.

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