Optimisation of Inulinase Production by Aspergillus niger Using Simplex and Classical Method

Marcin Skowronek and Jan Fiedurek*

Department of Industrial Microbiology, Maria Curie-Skłodowska University, Akademicka 19, PL-20-033 Lublin, Poland

Article history:
Received March 4, 2004
Accepted July 6, 2004

Key words:
simplex optimisation, inulinase, Aspergillus niger

Summary:
The simplex method has been used to optimise the inulinase production by Aspergillus niger strain 13/36 with respect to 5 components of the medium. The extracellular enzyme activity was almost 80 U/mL, and over 1.6-fold higher than the results obtained by the classical »one-factor-at-a-time« method. The greatest advantage of the simplex method is that the optimum factor levels were determined in only 12 iterative steps, shortening a typical optimum search process considerably. This is the first use of the simplex method for optimising inulinase production.

*Corresponding author:           This email address is being protected from spambots. You need JavaScript enabled to view it.
                                               ++48 81 53 75 959